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Drought stress can significantly affect plant growth and development. Biochar (BC) and plant growth-promoting rhizobacteria (PGPR) have been found to increase plant fertility and development under drought conditions. The single effects of BC and PGPR in different plant species have been widely reported under abiotic stress. However, there have been relatively few studies on the positive role of PGPR, BC, and their combination in barley (Hordeum vulgare L.). Therefore, the current study investigated the effects of BC from Parthenium hysterophorus, drought tolerant PGPR (Serratia odorifera), and the combination of BC + PGPR on the growth, physiology, and biochemical traits of barley plants under drought stress for two weeks. A total of 15 pots were used under five treatments. Each pot of 4 kg soil comprised the control (T0, 90% water), drought stress alone (T1, 30% water), 35 mL PGPR/kg soil (T2, 30% water), 2.5%/kg soil BC (T3, 30% water), and a combination of BC and PGPR (T4, 30% water). Combined PGPR and BC strongly mitigated the negative effects of drought by improving the shoot length (37.03%), fresh biomass (52%), dry biomass (62.5%), and seed germination (40%) compared to the control. The PGPR + BC amendment treatment enhanced physiological traits, such as chlorophyll a (27.9%), chlorophyll b (35.3%), and total chlorophyll (31.1%), compared to the control. Similarly, the synergistic role of PGPR and BC significantly (p< 0.05) enhanced the antioxidant enzyme activity including peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) to alleviate the toxicity of ROS. The physicochemical properties (N, K, P, and EL) of the soils were also enhanced by (85%, 33%, 52%, and 58%) respectively, under the BC + PGPR treatment compared to the control and drought stress alone. The findings of this study have suggested that the addition of BC, PGPR, and a combination of both will improve the soil fertility, productivity, and antioxidant defense systems of barley under drought stress. Therefore, BC from the invasive plant P. hysterophorus and PGPR can be applied to water-deficient areas to improve barley crop production.

Invasive alien plants pose severe threats to agroecosystems. Microplastic (MP) contamination in farmland soil is also concerning, as it causes crop stress and reduces productivity. However, the effects of the interactions between invasive alien plants and MP in the soil impact crops remain unclear. Herein, belowground plant characteristics associated with stress responses were examined in a pot experiment using root scan analyzes of rice plants exposed to Solidago canadensis L. invasion, polyethylene MP contamination, and a combined treatment. The observed changes in root growth traits under Canada goldenrod (Solidago canadensis L.) invasion were the least adverse, whereas S. canadensis invasion combined with soil MP contamination had the most adverse effects on root growth. Solidago canadensis L. invasion increased all belowground indices except root height and mean root diameter, which was upregulated in the soil MP contamination treatment. The combined treatment (S. canadensis invasion and soil MP contamination) reduced the belowground root growth traits more than the other treatments. The root growth traits may have been affected by changes in the antioxidant enzyme activity of the roots caused by the treatments. The combined effects of S. canadensis invasion and MP toxicity on rice root growth traits raise concerns regarding potential yields, financial damage, and consequences related to a potential move into the food web.

IntroductionBiochar (BC) and nitrogen (N) application have the potential to increase grain yield and resource use efficiency in intercropping systems. However, the effects of different levels of BC and N application in these systems remain unclear. To address this gap, the study is intended to ascertain the impact of various combinations of BC and N fertilizer on the performance of maize-soybean intercropping and determine the optimum application of BC and N for maximizing the effect of the intercropping system.MethodsA two-year (2021-2022) field experiment was conducted in Northeast China to assess the impact of BC (0, 15, and 30 t ha-1) and N application (135, 180, and 225 kg ha-1) on plant growth, yield, water use efficiency (WUE), N recovery efficiency (NRE) and quality in an intercropping system. Maize and soybean were selected as materials in the experiment, where every 2 rows of maize were intercropped with 2 rows of soybean.Results and discussionThe results showed that the combination of BC and N significantly affected the yield, WUE, NRE and quality of intercropped maize and soybean. The treatment of 15 t ha-1 BC and 180 kg ha-1 N increased grain yield and WUE, while that of 15 t ha-1 BC and 135 kg ha-1 N enhanced NRE in both years. Nitrogen promoted the protein and oil content of intercropped maize, but decreased the protein and oil content of intercropped soybean. BC did not enhance the protein and oil content of intercropped maize, especially in the first year, but increased maize starch content. BC was found to have no positive impact on soybean protein, but it unexpectedly increased soybean oil content. The TOPSIS method revealed that the comprehensive assessment value first increased and then declined with increasing BC and N application. BC improved the performance of maize-soybean intercropping system in terms of yield, WUE, NRE, and quality while N fertilizer input was reduced. The highest grain yield in two years was achieved for BC of 17.1-23.0 t ha-1 and N of 156-213 kg ha-1 in 2021, and 12.0-18.8 t ha-1 BC and 161-202 kg ha-1 N in 2022. These findings provide a comprehensive understanding of the growth of maize-soybean intercropping system and its potential to enhance the production in northeast China.

ObjectiveThis study investigated the prevalence of wrist injuries in 15 industries and different types of work in China. Study on the associated factors of wrist injuries provides a scientific basis for prevention and treatment of wrist diseases in occupational workers.MethodsA cross-sectional study of musculoskeletal symptoms of related practitioners in 15 industries, including automobile manufacturing, was conducted to retrieve worker demographic information, working wrist posture, and pain conditions. Multivariable binary logistic regression analyses were performed to identify factors associated with work-related musculoskeletal disorders (WMSDs).ResultsThe prevalence of wrist injuries among the study population was 13.2%. Toy manufacturing, animal husbandry, automobile manufacturing, shoe manufacturing, and biopharmaceutical manufacturing had the highest wrist injury rates at 29.1, 19.1, 14.9, 14.9, and 14.0%, respectively. Among the types of jobs, enamel workers (63.0%), butchers (43.6%), combers (32.5%), welders (31.3%), and scaffolders (26.5%) had the highest prevalence rates. Based on the final multivariate logistic regression analysis: female [odds ratios (OR) = 1.24; 95% confidence interval (CI), 1.15–1.35], 6–10 years of service (OR = 1.11; 95% CI, 1.03–1.18), >10 years of service (OR = 1.15; 95% CI, 1.06–1.25), frequent upward and downward flexion in wrist posture at work (OR = 1.81; 95% CI, 1.84–2.11), and frequent wrist placement on the edge of angular objects increased the OR of injury (OR = 1.52; 95% CI, 1.44–1.61). Need to squeeze objects tightly while working (OR = 1.72; 95% CI, 1.57–1.89), prolonged wrist flexion (OR = 1.86; 95% CI, 1.75–1.97), and work hand position above the shoulder for prolonged periods (OR = 1.11; 95% CI, 1.04–1.19) also suggested the relationship between these factors and the higher prevalence of wrist injury in the workers. The associated factor was physical activity (OR = 0.86; 95% CI, 0.80–0.94).ConclusionThis study suggested the relationship between these factors and the higher prevalence of wrist injury in the toy manufacturing, animal husbandry, automobile manufacturing, and shoe-making industries, enamel workers, butchers, and combers. And are work types that require special attention. Females, working age, physical activity, and abnormal posture of the wrist were factors significantly associated with WMSDs.

Invasive plants threaten biodiversity and cause huge economic losses. It is thought that global change factors (GCFs) associated with climate change (including shifts in temperature, precipitation, nitrogen, and atmospheric CO2) will amplify their impacts. However, only few studies assessed mixed factors on plant invasion. We collated the literature on plant responses to GCFs to explore independent, combined, and interactive effects on performance and competitiveness of native and invasive plants. From 176 plant species, our results showed that: (1) when native and invasive plants are affected by both independent and multiple GCFs, there is an overall positive effect on plant performance, but a negative effect on plant competitiveness; (2) under increased precipitation or in combination with temperature, most invasive plants gain advantages over natives; and (3) interactions between GCFs on plant performance and competitiveness were mostly synergistic or antagonistic. Our results indicate that native and invasive plants may be affected by independent or combined GCFs, and invasive plants likely gain advantages over native plants. The interactive effects of factors on plants were non-additive, but the advantages of invasive plants may not increase indefinitely. Our findings show that inferring the impacts of climate change on plant invasion from factors individually could be misleading. More mixed factor studies are needed to predict plant invasions under global change.

Changes in temperature and nitrogen (N) deposition determine the growth and competitive dominance of both invasive and native plants. However, a paucity of experimental evidence limits understanding of how these changes influence plant invasion. Therefore, we conducted a greenhouse experiment in which invasive Solidago canadensis L. was planted in mixed culture with native Artemisia argyi Levl. et Van under combined conditions of warming and N addition. Our results show that due to the strong positive effect of nitrogen addition, the temperature increases and nitrogen deposition interaction resulted in greatly enhanced species performance. Most of the relative change ratios (RCR) of phenotypic traits differences between S. canadensis and A. argyi occur in the low invasion stage, and six of eight traits had higher RCR in response to N addition and/or warming in native A. argyi than in invasive S. canadensis. Our results also demonstrate that the effects of the warming and nitrogen interaction on growth-related traits and competitiveness of S. canadensis and A. argyi were usually additive rather than synergistic or antagonistic. This conclusion suggests that the impact of warming and nitrogen deposition on S. canadensis can be inferred from single factor studies. Further, environmental changes did not modify the competitive relationship between invasive S. canadensis and native A. argyi but the relative yield of S. canadensis was significantly greater than A. argyi. This finding indicated that we can rule out the influence of environmental changes such as N addition and warming which makes S. canadensis successfully invade new habitats through competition. Correlation analysis showed that invasive S. canadensis may be more inclined to mobilize various characteristics to strengthen competition during the invasion process, which will facilitate S. canadensis becoming the superior competitor in S. canadensis-A. argyi interactions. These findings contribute to our understanding of the spreading of invasive plants such as S. canadensis under climate change and help identify potential precautionary measures that could prevent biological invasions.

This article explores the effects of abiotic stress on plants and the importance of studying how different stress responses interact. It discusses the use of advanced technologies to analyze the genetic and metabolic changes in plants under stress. The article presents various studies that examine the responses of different crops to stress, providing insights into how plants adapt and manage stress. These findings have implications for developing stress-resistant crops and improving crop tolerance and nutritional content. The studies also emphasize the interconnectedness of different stressors and their impact on plant physiology. [Extracted from the article]

Preeclampsia (PE) is a serious pregnancy-related disease, and patients usually present with a high inflammatory response. Previous studies have suggested that aspirin (ASP) may have a role in alleviating the pathogenesis of preeclampsia. However, whether ASP can improve kidney damage and the mechanism for improving it is currently unclear. Here we optimized a lipopolysaccharide (LPS)-induced PE mouse model to identify the role of ASP in renal protection. We found that ASP treatment ameliorated LPS-induced renal failure and pathological changes, the tubular injury was significantly attenuated by ASP. Administration of ASP decreased the renal expression of pro-inflammatory factors, resulting in reduced kidney inflammation. The number of GALECTIN-3-positive cells was reduced, and the up-regulation of IL-6 and TNF-α was decreased. In addition, ASP also suppressed renal cell apoptosis and oxidative stress. An in vitro study indicated that ASP relieved LPS-induced HK-2 cell damage by inhibiting WNT5A/NF-κB signaling. Collectively, our data suggest that ASP is a useful therapeutic option for PE-related kidney injury.

In this study, we evaluated the performance of three blood culture systems in a Chinese tertiary-care hospital. Samples of simulated bacteremia were prepared using 10 mL of fresh blood from healthy humans and bacterial suspensions of known cell density. Portions of the specimens were treated with an antibiotic or antifungal drug at specified concentrations to simulate antibacterial drug treatment. We analyzed three blood culture systems: BACTEC Plus, BacT/Alert, and VersaTREK. Both time-to-detection (TTD) of 10 types of bacteria and five types of yeasts in samples without antibiotic treatment and positive detection rate of samples treated with different concentrations of antibiotic or antifungal drugs were compared among the culture systems. We also retrospectively analyzed the use of the culture systems in our hospital from 2015 to 2018. In the simulated study, in the absence of antibiotics, the VersaTREK REDOX 1 displayed the shortest TTD for Pseudomonas aeruginosa, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Candida albicans, and Candida glabrata (P < 0.001). Among the anaerobically cultured samples, BACTEC lytic/10 anaerobic/F displayed the shortest TTD for Escherichia coli, S. aureus, Enterococcus faecalis, S. pneumoniae, Bacteroides fragilis, and Bacteroides vulgatus (P < 0.001). Comparatively, BacT/Alert FA/FN showed no advantages. In antibiotic-treated samples, overall recovery rates for the BACTEC, BacT/Alert, and VersaTREK systems were, were 70.2, 43.7, and 27.4%, respectively. BACTEC facilitated higher recovery rate than the other two systems (P < 0.001). In antifungal treatment, the overall recovery rates for the BACTEC, BacT/Alert, and VersaTREK systems were 93.9, 98.3, and 69.4%, respectively. BACTEC Plus showed a recovery rate comparable to that of BacT/Alert (P = 0.835), and the recovery rate of both these systems was higher than that of VersaTREK (P < 0.001). The TTD values and positive rates determined in the retrospective study were consistent with those obtained in the simulated study. The combination of BACTEC PLUS Aerobic/F and BACTEC lytic/10 anaerobic/F culture systems displayed the best clinical performance. Furthermore, the BacT/Alert FAN culture system was found to be more resistant to antifungal drugs and levofloxacin, whereas the VersaTREK system is considered more suitable for primary blood cultures without antibiotic supplementation.

Drought stress can significantly affect plant growth and development. Biochar (BC) and plant growth-promoting rhizobacteria (PGPR) have been found to increase plant fertility and development under drought conditions. The single effects of BC and PGPR in different plant species have been widely reported under abiotic stress. However, there have been relatively few studies on the positive role of PGPR, BC, and their combination in barley (Hordeum vulgare L.). Therefore, the current study investigated the effects of BC from Parthenium hysterophorus, drought tolerant PGPR (Serratia odorifera), and the combination of BC + PGPR on the growth, physiology, and biochemical traits of barley plants under drought stress for two weeks. A total of 15 pots were used under five treatments. Each pot of 4 kg soil comprised the control (T0, 90% water), drought stress alone (T1, 30% water), 35 mL PGPR/kg soil (T2, 30% water), 2.5%/kg soil BC (T3, 30% water), and a combination of BC and PGPR (T4, 30% water). Combined PGPR and BC strongly mitigated the negative effects of drought by improving the shoot length (37.03%), fresh biomass (52%), dry biomass (62.5%), and seed germination (40%) compared to the control. The PGPR + BC amendment treatment enhanced physiological traits, such as chlorophyll a (27.9%), chlorophyll b (35.3%), and total chlorophyll (31.1%), compared to the control. Similarly, the synergistic role of PGPR and BC significantly (p< 0.05) enhanced the antioxidant enzyme activity including peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) to alleviate the toxicity of ROS. The physicochemical properties (N, K, P, and EL) of the soils were also enhanced by (85%, 33%, 52%, and 58%) respectively, under the BC + PGPR treatment compared to the control and drought stress alone. The findings of this study have suggested that the addition of BC, PGPR, and a combination of both will improve the soil fertility, productivity, and antioxidant defense systems of barley under drought stress. Therefore, BC from the invasive plant P. hysterophorus and PGPR can be applied to water-deficient areas to improve barley crop production. [ABSTRACT FROM AUTHOR]