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Driven by the development of the oil and gas sector, Qatar has made remarkable economic achievements over the past few decades. However, Qatar’s high dependence on fossil fuels has resulted in a rapid increase in its CO2 emissions. As such, the country may be facing a trade-off between achieving continued economic growth and carbon emissions reductions. This study empirically analyzes the relationship between economic growth and carbon emissions in Qatar, using annual data of income and CO2 emissions from 1970 to 2018. The analysis was conducted by employing the Environmental Kuznets Curve (EKC) framework and the Decoupling Index (DI) approach. The EKC was evaluated using the Fully Modified Ordinary Least Squares cointegration method. Additionally, a robustness check was conducted by Canonical Cointegrating Regression (CCR) cointegration method to confirm the results attained by FMOLS. The findings support the evidence of an N-shaped relationship and suggest that the country is currently undergoing expanding relative decoupling. Although findings show that Qatar’s per capita emissions have been declining over the last few years, additional measures such as energy conservation, energy efficiency and renewable energy are required to allow cost-effective carbon emission reductions.

Inherited retinal dystrophies (IRDs) are a large and heterogeneous group of degenerative diseases caused by mutations in various genes. Given the favorable anatomical and immunological characteristics of the eye, gene therapy holds great potential for their treatment. Our goal is to validate the preservation of visual functions by viral-free homology directed repair (HDR) in an autosomal recessive loss of function mutation. We used a tailored gene editing system based on clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) to prevent retinal photoreceptor death in the retinal degeneration 10 (Rd10) mouse model of retinitis pigmentosa. We tested the gene editing tool in vitro and then used in vivo subretinal electroporation to deliver it to one of the retinas of mouse pups at different stages of photoreceptor differentiation. Three months after gene editing, the treated eye exhibited a higher visual acuity compared to the untreated eye. Moreover, we observed preservation of light-evoked responses both in explanted retinas and in the visual cortex of treated animals. Our study validates a CRISPR/Cas9-based therapy as a valuable new approach for the treatment of retinitis pigmentosa caused by autosomal recessive loss-of-function point mutations.

The corticotectal projections, together with the corticobulbar (corticoreticular) projections, work in parallel with the corticospinal tract (CST) to influence motoneurons in the spinal cord both directly and indirectly via the brainstem descending pathways. The tectospinal tract (TST) originates in the deep layers of the superior colliculus. In the present study, we analyzed the corticotectal projections from two motor cortical areas, namely the premotor cortex (PM) and the primary motor cortex (M1) in eight macaque monkeys subjected to either a cortical lesion of the hand area in M1 (n = 4) or Parkinson’s disease-like symptoms PD (n = 4). A subgroup of monkeys with cortical lesion was subjected to anti-Nogo-A antibody treatment whereas all PD monkeys were transplanted with Autologous Neural Cell Ecosystems (ANCEs). The anterograde tracer BDA was used to label the axonal boutons both en passant and terminaux in the ipsilateral superior colliculus. Individual axonal boutons were charted in the different layers of the superior colliculus. In intact animals, we previously observed that corticotectal projections were denser when originating from PM than from M1. In the present M1 lesioned monkeys, as compared to intact ones the corticotectal projection originating from PM was decreased when treated with anti-Nogo-A antibody but not in untreated monkeys. In PD-like symptoms’ monkeys, on the other hand, there was no consistent change affecting the corticotectal projection as compared to intact monkeys. The present pilot study overall suggests that the corticotectal projection is less affected by M1 lesion or PD symptoms than the corticoreticular projection previously reported in the same animals.

Overtopping breakwater systems are among the most promising technologies for exploiting wave energy to generate electricity. They consist in water reservoirs, embedded in piers, placed on top of ramps, higher than sea-level. Pushed by wave energy, seawater fills up the reservoirs and produces electricity by flowing back down through low headhydro turbines. Different overtopping breakwater systems have been tested worldwide in recent years. This study focuses on the Overtopping BReakwater for Energy Conversion (OBREC) system that has been implemented and tested in the harbor of Naples (Italy). The Life Cycle Assessment of a single replicable module of OBREC has been performed for analyzing potential environmental impacts, in terms of Greenhouse Gas Emissions, considering construction, installation, maintenance, and the operational phases. The Carbon Footprint (i.e., mass of CO2eq) to build wave energy converters integrated in breakwater systems has been estimated, more specifically the “environmental investment” (i.e., the share of Carbon Footprint due to the integration of wave energy converter) needed to generate renewable electricity has been assessed. The Carbon Intensity of Electricity (i.e., the ratio between the CO2eq emitted and the electricity produced) has been then assessed in order to demonstrate the profitability and the opportunity to foster innovation in the field of blue energy. Considering the impact for implementing an operational OBREC module (Carbon Footprint = 1.08 t CO2eq; Environmental Investment = 0.48 t CO2eq) and the electricity production (12.6 MWh/year per module), environmental benefits (avoided emissions) would compensate environmental costs (i.e., Carbon Footprint; Environmental Investment) those provided within a range of 25 and 13 months respectively.

The opportunity to co-locate wind and wave energy exploitation is analyzed in the Italian seas grounding on the rationale that benefits are greater when un-correlated resources are combined. The study shows that, although waves and winds are generally strongly correlated, in some conditions their correlation is lower and the combined energy harvesting more interesting. As spatial conflicts of sea use and demand for maritime space are increasing, the development of the marine renewable energy sector needs to be evaluated in the perspective of the cumulative pressures deriving from present activities or expected from future developments. The evaluation of areas of potential conflicts among human activities, environmental vulnerabilities and marine renewable developments may facilitate the early development of mitigation actions and negotiations between stakeholders. In this study the opportunity of co-locating offshore wind turbines and wave energy converters is analyzed through a spatial planning approach. Both the potential for combining different renewable technologies, and the impact associated to such development was considered in the context of the existing pressures (e.g., naval traffic; mariculture activities; submarine cables routes; dredge spoils dumping; offshore activities; windfarms and ocean energy projects) and vulnerabilities (Marine Protected Areas, Key habitat presence) through quantitative indicators. The portion of Tyrrhenian coast south of Elba island, the northern-western Sardinian coast, and the southern Adriatic and Ionian coastal waters appear to be the most suitable sites. Moreover, the study presents a spatial quantitative methodology to identify sites of potential interest for the development of the marine renewable energy sector in the perspective of cost-effectiveness and environmental impact minimization.

Plants, algae and most bacteria synthesize 5-aminolevulinic acid (ALA), the universal precursor of tetrapyrroles such as heme, chlorophyll and coenzyme B12, by a two-step transformation involving the NADPH-dependent glutamyl-tRNA reductase (HemA), which reduces tRNA-bound glutamate to glutamate-1-semialdehyde (GSA), and the pyridoxamine 5′-phosphate-dependent glutamate-1-semialdehyde-2,1-aminomutase (HemL), responsible for the isomerization of GSA into ALA. Since GSA is a very unstable compound at pH values around neutrality, the formation of a HemA-HemL complex has been proposed to occur, allowing for direct channeling of this intermediate from HemA to HemL. Experimental evidence of the formation of this complex has been obtained with the enzymes from Escherichia coli and Chlamydomonas reinhardtii. However, its isolation has never been attained, probably because HemA is degraded when intracellular heme accumulates. In this work, we devised a co-expression and co-purification strategy of HemA and HemL from Acinetobacter baumannii, which allowed the isolation of the HemA-HemL complex. Our results indicate that HemA is stabilized when co-expressed with HemL. The addition of citrate throughout the expression and purification procedure further promotes the formation of the HemA-HemL complex, which can be isolated in fair amount for functional and structural studies. This work lays the bases for a rational design of HemA-HemA inhibitors to be developed as antibacterial agents against A. baumannii, a multidrug resistant opportunistic pathogen responsible for a broad range of severe nosocomial infections.

Haploinsufficiency of the SHANK3 gene, encoding for a scaffolding protein located in the postsynaptic density of glutamatergic synapse, has been linked to forms of autism spectrum disorders (ASDs). It has been shown that SHANK3 controls the maturation of social reward circuits in the ventral tegmental area (VTA). Whether the impairments in associative learning observed in ASD relate to SHANK3 insufficiency restricted to the reward system is still an open question. Here, we first characterize a social-conditioned place preference (CPP) paradigm based on the direct and free interaction with a juvenile and non-familiar conspecific. In both group- and single-housed C57Bl6/j late adolescence male mice, this CPP protocol promotes the formation of social-induced contextual associations that undergo extinction. Interestingly, the downregulation of Shank3 expression in the VTA altered the habituation to a non-familiar conspecific during conditioning and accelerated the extinction of social-induced conditioned responses. Thus, inspired by the literature on drugs of abuse-induced contextual learning, we propose that acquisition and extinction of CPP might be used as behavioral assays to assess social-induced contextual association and “social-seeking” dysfunctions in animal models of psychiatric disorders.