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IntroductionThe cognitive abilities of animals play a crucial role in their capacity to adapt to changing environments. Experiments to understand cognition are usually performed on animals such as non-human primates, canids, or corvids. These studies often use food as the motivating factor. However, in the current study, we used a new paradigm to investigate the ability of ponerine ants, who are solitary foragers, to solve a nature-inspired problem. As pupae are the most invested brood item and are directly associated with the colony’s fitness, we designed an experiment that presented a challenge to colony relocation by imposing restrictions on the transportation of pupae to the new nest.MethodsWe used a narrow nest entrance through which an adult could enter, but it rendered the passage of an adult holding a pupa virtually impossible. Through this, we examined the capacity of these ants to overcome the “pupal insertion” challenge by investigating eight colonies involving 425 pupal insertions performed by 104 uniquely labeled ants. ResultsWe found that 80% of all pupae were inserted into the nest by employing a novel cooperative behavior: one ant pushed the pupa while the other ant(s) inside the nest pulled it. Even though individual ants showed high variability in their performance regarding pupal insertion, the initial three successive insertions showed a significant improvement in their performance. Such improvement was also documented at the colony level, with shorter durations and fewer attempts for subsequent pupal insertions. DiscussionOur study demonstrated that ants can overcome difficult environmental challenges through innovation and subsequently improve their performance without training or reward.