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IntroductionResearch on work-family dynamics during the COVID-19 pandemic shows that family demands increased for workers, particularly those with children. This heightened family-to-work conflict negatively affects the subjective well-being of parents and their children. However, these outcomes have been mainly examined on individuals without considering the family as the unit of analysis. This study aimed to explore the relationships between family-to-work conflict, psychological distress, and family life satisfaction in dual-earner parents with adolescent children during the second year of the COVID-19 pandemic. Moreover, the potential mediating role of psychological distress among FtoWC and family life satisfaction and the moderating role of household monthly income were tested.MethodsThe non-probabilistic sample in Chile comprised 860 dual-earner parents and one of their adolescent children (mean age 13.5 years, 50.8% female). Parents answered an online questionnaire with FtoWC scale, whereas parents and adolescents answered the Depression, Anxiety, Stress Scale and the Satisfaction with Family Life Scale. Analysis was conducted using structural equation modeling and the mediation actor-partner interdependence model.ResultsFtoWC was directly linked to lower family life satisfaction in fathers and via a mediating role of psychological distress in both parents. Mothers’ FtoWC was related via the mediating role of the fathers’ psychological distress to fathers’ lower family life satisfaction and via the mediating role of the adolescents’ psychological distress to adolescents’ lower family life satisfaction. Monthly household income moderated three APIM model paths involving mothers’ variables.DiscussionThese findings underscore the importance of implementing family-oriented workplace policies. Such policies may help mitigate both parents’ experiences of FtoWC and their resulting psychological distress.

IntroductionAssessment of the Comprehensive Feeding Practices Questionnaire in adolescents (CFPQ-Teen) is still limited, with no evaluation of the measurement invariance. The participants comprised 473 Chilean adolescents of both sexes from dual-income nuclear families. The aims of this study were: (1) to adapt to Spanish and validate a model of five-factor version the CFPQ-Teen; (2) to examine the psychometric properties, (3) to evaluate the measurement invariance according to the adolescents’ gender; and (4) to compare the scores of each factor between female and male adolescents.MethodsThe instrument was translated, back-translated, and adapted from the CFPQ-Teen, confirming the equivalence, conceptual, and face validity in a pilot sample of 40 adolescents. An exploratory factor analysis was performed on the five-factor model of the CFPQ-Teen: Monitoring, Adolescent Control, Restriction for weight control, Parental Modeling, and Environment. The Environment factor was eliminated as a result.ResultsThe confirmatory factor analysis presented good reliability, convergent, discriminant, and concurrent validity values. In addition, medium to high goodness-of-fit levels were obtained by eliminating an item from the Adolescent Control factor. These results confirm a final 20-item model representing four factors. The multigroup invariance analysis of the measurement model verified configural, metric, scalar, and partial strict invariance. No significant differences were found between females and males in the scores on the four factors.DiscussionThese results enable comparisons by sex on the perceptions of Food Parenting Practices from the analyzed factors, primarily within the context of the Chilean sample.

Microgreens are vegetable or edible herb shoots harvested in the early stages of development. They have an important number of bioactive compounds and add color, texture, and flavor to dishes and salads. Given their benefits, small size, and high market prices, they can grow in indoor systems, where light is determinant. This study aimed to evaluate the effect of different light intensities on agronomic characteristics, color, chlorophylls and carotenoids content, and antioxidant activity represented by total phenolic content (TPC), eliminate, and antioxidant capacity (AC) in four Brassicaceae species in two colors (green and red). The experiment was conducted in a controlled light-emitting diode (LED) environment growth chamber (day/night temperatures of 25/20 ± 1.2°C, 16 h photoperiod, and 79 ± 2% relative humidity). Three light intensities were used for microgreen growth with the same LED light spectrum: low (120 ± 5.1 μmol m−2 s−1), medium (160 ± 3.6 μmol m−2 s−1), and high (210 ± 5.9 μmol m−2 s−1). Eight g of the seeds of green and red cultivars of cabbage, kale, mizuna, and mustard were sown in a plastic tray (64 cm x 35 cm x 6 cm) with a mixture of peat and perlite (1:2 = v: v). Overall, the high intensity increased dry matter percentage and dry weight, except in green and red kale and green cabbage cultivars. In contrast, low intensity promoted a larger hypocotyl in all species than with high intensity; moreover, it enhanced the cotyledon area in green and red mizuna. Cabbage, kale, and mustard green cultivars were greener under medium intensity, whereas the low intensity enhanced the purple color of mizuna. In addition, chlorophyll a and b increased under low intensity in most species except the red kale and mustard cultivars. The high intensity raises the antioxidant activity, promoting a higher TPC and AC. The findings revealed that the light intensity generated variations in agronomic characteristics, color, chlorophyll content, and antioxidant activity of Brassicaceae microgreens, and the changes were based on the specific species and cultivars.

Understanding the spatial structure of fishing resource stocks is a fundamental aspect for the management and development of sustainable and productive fisheries. However, this aspect is usually simplified under the assumption of a stock unit, which assumes biologically similar and spatially homogeneous individuals, regardless of the real ranges and characteristics of their distribution. The persistence of geographic patterns in the biological traits of species suggests the spatial structuring of a population, a highly relevant aspect in stock assessment, calculation of Biological Reference Points (BRP), and management of fishing resources. In this work, the spatial heterogeneity of the biological traits of common hake (Merluccius gayi) from off-central Chile is modeled. Considering four reference zones, biological data collected over 26 years of monitoring fisheries and acoustic surveys is analyzed. The findings allow to biologically differentiate these areas whose main characteristics are the increase from north to south in the condition factor, gonad weight, and size at maturity. The Spawning Potential Ratio (SPR), fishing mortality, and BRP are calculated. Exploratorily, if these areas were considered closed subpopulations, results show that overexploitation is not a characteristic of all hake fishing areas, and that not considering the heterogeneity of biological traits causes the overestimation of the population condition in the main fishing areas. The aim of this research was to understand the spatial structuring of common hake based on the heterogeneity of biological attributes and its impact for fisheries management purposes.

IntroductionOlder adults are a highly heterogeneous population, as individuals of the same age can show considerable variations in personal characteristics and living conditions. Risk and protective factors for older adults' subjective wellbeing during the COVID-19 pandemic can be explored by examining how life satisfaction, food-related life satisfaction, and associated variables coexist among these individuals. On this basis, this study aimed to identify older adult profiles based on their levels of life and food-related life satisfaction; to characterize these profiles by diet quality, social support, financial wellbeing, and sociodemographic characteristics; and to identify variables associated with higher life and food-related life satisfaction.MethodsThe sample included 1,371 institutionalized and non-institutionalized individuals over the age of 60, from four cities in Chile. Participants answered a survey, either online or face to face, with questions about life and food-related life satisfaction, perceived social support from family, friends, and others, food quality, financial wellbeing/distress, sociodemographic characteristics, and prior COVID-19 infection.ResultsUsing a latent profile analysis, we identified three profiles of older adults: Profile 1: Unsatisfied with their life, somewhat satisfied with their food-related life (5.40%); Profile 2: Somewhat satisfied with their life, satisfied with their food-related life (65.06%); Profile 3: Extremely satisfied with their life and food-related life (29.54%). Profiles differed by residence (institutionalized vs. independent), age, marital status, social support, financial wellbeing, COVID-19 infection, and city of residence.DiscussionThe patterns of association between life and food-related satisfaction and related variables indicate conditions of vulnerability and protection related to living conditions, the social dimensions of food consumption, and social support. These results underscore the need for identifying groups of older adults based on diverse characteristics and conditions outside of chronological age.

Introduction: Neuronal Ca2+ signals generated through the activation of Ca2+-induced Ca2+ release in response to activity-generated Ca2+ influx play a significant role in hippocampal synaptic plasticity, spatial learning, and memory. We and others have previously reported that diverse stimulation protocols, or different memory-inducing procedures, enhance the expression of endoplasmic reticulum-resident Ca2+ release channels in rat primary hippocampal neuronal cells or hippocampal tissue.Methods and Results: Here, we report that induction of long-term potentiation (LTP) by Theta burst stimulation protocols of the CA3-CA1 hippocampal synapse increased the mRNA and protein levels of type-2 Ryanodine Receptor (RyR2) Ca2+ release channels in rat hippocampal slices. Suppression of RyR channel activity (1 h preincubation with 20 μM ryanodine) abolished both LTP induction and the enhanced expression of these channels; it also promoted an increase in the surface expression of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits GluR1 and GluR2 and caused a moderate but significant reduction of dendritic spine density. In addition, training rats in the Morris water maze induced memory consolidation, which lasted for several days after the end of the training period, accompanied by an increase in the mRNA levels and the protein content of the RyR2 channel isoform.Discussion: We confirm in this work that LTP induction by TBS protocols requires functional RyR channels. We propose that the increments in the protein content of RyR2 Ca2+ release channels, induced by LTP or spatial memory training, play a significant role in hippocampal synaptic plasticity and spatial memory consolidation.

IntroductionResearch has evaluated the impact of COVID-19 pandemic and lockdowns on individuals' life satisfaction, but wellbeing interrelations between family members in this context have been less explored. This study examined the spillover and crossover effects of one parent's job satisfaction (JS), satisfaction with family life (SWFaL) and satisfaction with food-related life (SWFoL) on their own, their partner's, and their adolescent children's life satisfaction (LS), and the influence of adolescents' SWFaL and SWFoL on their own and their parents' LS, in dual-earner families with adolescents. The moderating role of job-related variables of both parents were also explored.MethodsQuestionnaires were administered to 860 dual-earner parents with adolescents in two cities in Chile during 2020. Mothers and fathers answered the Overall Job Satisfaction Scale and the three family members answered the Satisfaction with Life Scale, the Satisfaction with Family Life Scale and the Satisfaction with Food-related Life Scale.ResultsUsing the Actor-Partner Interdependence Model and structural equation modeling, we found that fathers' LS was positively associated with their own JS, SWFaL and SWFoL (spillover), and negatively with adolescents' SWFoL (crossover). Likewise, mothers' LS was positively associated with their own JS, SWFaL and SWFoL (spillover), with fathers' and adolescents' SWFaL, and negatively with adolescents' SWFoL. Adolescents' LS was positively associated with their own SWFaL and SWFoL (spillover), and with their fathers' JS, and negatively with their fathers' SWFoL. JS showed gendered patterns in spillover and crossover associations. Parents' type of employment, mothers' working hours and city of residence moderated some spillover and crossover associations for father-mother and parent-adolescent dyads.DiscussionThese findings suggest that, for dual-earner parents with adolescents, improving individuals' LS requires interventions that should be carried out not individually, but at a family level.

Reciprocal family influences in the food domain have been little explored, particularly during the COVID-19 pandemic. To fill in this gap, this study explored actor and partner effects between parents’ food modeling and parents’ and their adolescent children’s diet quality and satisfaction with food-related life (SWFoL); and the mediating role of diet quality between modeling and SWFoL. This study used a cross-sectional design. A sample of 430 different-sex dual-earner parents and one adolescent child were recruited in Rancagua, Chile, between March and June 2020. Parents answered the modeling dimension of the Comprehensive Feeding Practices Questionnaire. Parents and adolescents answered the Adapted Healthy Eating Index (AHEI) and the SWFoL Scale. Analyses were conducted using the Actor-Partner Interdependence Model and structural equation modeling. Results showed that one parent’s modeling enhanced diet quality for themselves, their partner, and the adolescents. Parents’ modeling was associated with their own SWFoL, directly and via their own diet quality. There were positive associations between mothers’ modeling and adolescents’ SWFoL; between mothers’ diet quality and fathers’ SWFoL; and between mothers’ modeling and fathers’ SWFoL via the fathers’ diet quality. Parents’ modeling can improve the three family members’ diet quality, while mothers’ modeling and diet quality showed to improve fathers’ and adolescents’ SWFoL.

The impact of work-to-family conflict (WtoFC) can extend beyond family and work, and to other domains that contribute to well-being, such as the food domain. This study examined associations between WtoFC, perception of atmosphere of family meals (AFM), and satisfaction with food-related life (SWFoL) in dual-earner parents with adolescent children, and tested the mediating role of AFM between WtoFC and SWFoL. Questionnaires were administered to 473 different-sex dual-earner parents and one of their adolescent children (mean age 12.5 years, 51.4% male) in Temuco, Chile. Parents responded to a measure of work-to-family conflict; the three family members answered the Project-EAT Atmosphere of family meals scale, and the Satisfaction with Food-related Life Scale. Analyses were conducted using the Actor-Partner Interdependence Model and structural equation modeling. Results showed a negative association from WtoFC to SWFoL in both parents, while a more positive perception of atmosphere of family meals was linked to higher SWFoL in the three family members. Moreover, WtoFC was negatively linked to SWFoL in parents, while only mothers' WtoFC had a negative association with their adolescent children's SWFoL. Policymakers and organizations can contribute to workers' and their families' food-related well-being by fostering policies and measures to reduce WtoFC.

Triclosan, a widely used industrial and household agent, is present as an antiseptic ingredient in numerous products of everyday use, such as toothpaste, cosmetics, kitchenware, and toys. Previous studies have shown that human brain and animal tissues contain triclosan, which has been found also as a contaminant of water and soil. Triclosan disrupts heart and skeletal muscle Ca2+ signaling, damages liver function, alters gut microbiota, causes colonic inflammation, and promotes apoptosis in cultured neocortical neurons and neural stem cells. Information, however, on the possible effects of triclosan on the function of the hippocampus, a key brain region for spatial learning and memory, is lacking. Here, we report that triclosan addition at low concentrations to hippocampal slices from male rats inhibited long-term potentiation but did not affect basal synaptic transmission or paired-pulse facilitation and modified the content or phosphorylation levels of synaptic plasticity-related proteins. Additionally, incubation of primary hippocampal cultures with triclosan prevented both the dendritic spine remodeling induced by brain-derived neurotrophic factor and the emergence of spontaneous oscillatory Ca2+ signals. Furthermore, intra-hippocampal injection of triclosan significantly disrupted rat navigation in the Oasis maze spatial memory task, an indication that triclosan impairs hippocampus-dependent spatial memory performance. Based on these combined results, we conclude that triclosan exerts highly damaging effects on hippocampal neuronal function in vitro and impairs spatial memory processes in vivo.

Amyloid β peptide oligomers (AβOs), toxic aggregates with pivotal roles in Alzheimer’s disease, trigger persistent and low magnitude Ca2+ signals in neurons. We reported previously that these Ca2+ signals, which arise from Ca2+ entry and subsequent amplification by Ca2+ release through ryanodine receptor (RyR) channels, promote mitochondrial network fragmentation and reduce RyR2 expression. Here, we examined if AβOs, by inducing redox sensitive RyR-mediated Ca2+ release, stimulate mitochondrial Ca2+-uptake, ROS generation and mitochondrial fragmentation, and also investigated the effects of the antioxidant N-acetyl cysteine (NAC) and the mitochondrial antioxidant EUK-134 on AβOs-induced mitochondrial dysfunction. In addition, we studied the contribution of the RyR2 isoform to AβOs-induced Ca2+ release, mitochondrial Ca2+ uptake and fragmentation. We show here that inhibition of NADPH oxidase type-2 prevented the emergence of RyR-mediated cytoplasmic Ca2+ signals induced by AβOs in primary hippocampal neurons. Treatment with AβOs promoted mitochondrial Ca2+ uptake and increased mitochondrial superoxide and hydrogen peroxide levels; ryanodine, at concentrations that suppress RyR activity, prevented these responses. The antioxidants NAC and EUK-134 impeded the mitochondrial ROS increase induced by AβOs. Additionally, EUK-134 prevented the mitochondrial fragmentation induced by AβOs, as previously reported for NAC and ryanodine. These findings show that both antioxidants, NAC and EUK-134, prevented the Ca2+-mediated noxious effects of AβOs on mitochondrial function. Our results also indicate that Ca2+ release mediated by the RyR2 isoform causes the deleterious effects of AβOs on mitochondrial function. Knockdown of RyR2 with antisense oligonucleotides reduced by about 50% RyR2 mRNA and protein levels in primary hippocampal neurons, decreased by 40% Ca2+ release induced by the RyR agonist 4-chloro-m-cresol, and significantly reduced the cytoplasmic and mitochondrial Ca2+ signals and the mitochondrial fragmentation induced by AβOs. Based on our results, we propose that AβOs-induced Ca2+ entry and ROS generation jointly stimulate RyR2 activity, causing mitochondrial Ca2+ overload and fragmentation in a feed forward injurious cycle. The present novel findings highlight the specific participation of RyR2-mediated Ca2+ release on AβOs-induced mitochondrial malfunction.

Recognition memory comprises recollection judgment and familiarity, two different processes that engage the hippocampus and the perirhinal cortex, respectively. Previous studies have shown that aged rodents display defective recognition memory and alterations in hippocampal synaptic plasticity. We report here that young rats efficiently performed at short-term (5 min) and long-term (24 h) hippocampus-associated object-location tasks and perirhinal cortex-related novel-object recognition tasks. In contrast, aged rats successfully performed the object-location and the novel-object recognition tasks only at short-term. In addition, aged rats displayed defective long-term potentiation (LTP) and enhanced long-term depression (LTD). Successful long-term performance of object-location but not of novel-object recognition tasks increased the protein levels of ryanodine receptor types-2/3 (RyR2/RyR3) and of IP3R1 in young rat hippocampus. Likewise, sustained LTP induction (1 h) significantly increased RyR2, RyR3 and IP3R1 protein levels in hippocampal slices from young rats. In contrast, LTD induction (1 h) did not modify the levels of these three proteins. Naïve (untrained) aged rats displayed higher RyR2/RyR3 hippocampal protein levels but similar IP3R1 protein content relative to young rats; these levels did not change following exposure to either memory recognition task or after LTP or LTD induction. The perirhinal cortex from young or aged rats did not display changes in the protein contents of RyR2, RyR3, and IP3R1 after exposure at long-term (24 h) to the object-location or the novel-object recognition tasks. Naïve aged rats displayed higher RyR2 channel oxidation levels in the hippocampus compared to naïve young rats. The RyR2/RyR3 up-regulation and the increased RyR2 oxidation levels exhibited by aged rat hippocampus are likely to generate anomalous calcium signals, which may contribute to the well-known impairments in hippocampal LTP and spatial memory that take place during aging.