1. Association of Carbapenemase-Producing Enterobacterales Detected in Stream and Clinical Samples.
- Author
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Sung, Gyung-Hye, Kim, Si Hyun, Park, Eun Hee, Hwang, Suk Nam, Kim, Jea-Dong, Kim, Gyu Ri, Kim, Eun-Young, Jeong, Joseph, Kim, Sunjoo, and Shin, Jeong Hwan
- Subjects
ENTEROBACTERIACEAE ,KLEBSIELLA pneumoniae ,CITROBACTER freundii ,ENTEROBACTER cloacae ,CARBAPENEMASE ,TREATMENT failure ,WATER sampling ,BETA lactamases - Abstract
Background: The spread of carbapenem-resistant Enterobacterales (CRE) strains has caused treatment failure and is a worldwide threat to public health. However, there are limited reports on the prevalence of carbapenemase-producing Enterobacterales (CPE) in aquatic environments and its association with clinical isolates. This study aimed to investigate the prevalence of CPE in a stream environment and its genetic relationship with clinical isolates in Korea. Methods: A total of 4,582 water samples were collected from 94 streams. Multiplex PCR and sequencing were used to detect and identify six carbapenemase genes. Multi-locus sequence typing (MLST) was performed to investigate the genetic relatedness between the environmental strains and clinical isolates. Results: A total of 133 CRE strains were isolated from the streams. Klebsiella pneumoniae was the most common CRE (45.9%), followed by Enterobacter cloacae complex (29.3%), Escherichia coli (13.5%), Raoultella ornithinolytica (5.3%), and Citrobacter freundii (2.3%). Ninety (67.7%) isolates carried carbapenemase genes. K. pneumoniae carbapenemase-2 (36.7%) and New Delhi metallo-β-lactamase-5 (32.2%) were the common carbapenemases detected. Sequence type (ST)307 and ST11 K. pneumoniae strains harboring the bla
KPC-2 gene were the most prevalent in stream and patient samples. Conclusion: CPE was highly prevalent in streams and closely related to the isolates obtained from patients. Therefore, continuous monitoring of stream environments is required to control the spread of carbapenem resistance. [ABSTRACT FROM AUTHOR]- Published
- 2022
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