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In order to understand the effect of low - frequency vibration stimulation on the behavior and physiology of Larimichthys crocea,this study simulated the vibration of breeding cabin by self-made steel sink and actuator,and used the main frequency of underwater noise of traditional knocking operation to catch large yellow croaker. The frequency of 125 Hz (150 dB) and 200 Hz (150 dB) were used to conduct vibration stimulation experiments on 250 g and 500 g groups of large yellow croaker to determine the behavioral response and physiological and biochemical changes of large yellow croaker ( cortisol, epinephrine, thyroxine). The results showed that:(1) In the early stage of acoustic stimulation,the behavioral responses of the two groups of test fish were characterized by random movement,cruising away after slow approach,free or around cruising. After adaptation,the test fish were basically in a regular or free cruising around the wall,cruising away after slow approach,or stagnant,and there was no obvious disturbance response. (2) In 250 g test group,the change of adrenaline at 125 Hz frequency (about 40. 93% increase) was the most significant (P<0. 05). Under the condition of 200 Hz frequency,the change of thyroxine (increased by about 41. 08%) was the most significant (P< 0. 05). Except for increase of other blood indexes except epinephrine was higher than that of 125 Hz frequency group. Therefore,250 g large yellow croaker was more sensitive to 200 Hz. In the 500 g test group, the change of thyroxine ( increased by about 28. 68%) at 125 Hz frequency was the most significant ( P < 0. 05),and other blood indexes decreased. Under the condition of 200 Hz frequency,the change of thyroxine (increased by about 41. 79%) was the most significant (P<0. 05) and higher than that of 125 Hz frequency group,while other blood indexes increased in different degrees in 200 Hz frequency group. Therefore,the 250 g and 500 g groups were more sensitive to 200 Hz vibration stimulation. In this study, the effects of low - frequency vibration stimulation on the behavioral response and physiological and biochemical indexes of large yellow croaker were analyzed. The aim was to improve the growth rate,survival rate and quality of large yellow croaker during the breeding process of large yellow croaker,and to provide data support and reference [ABSTRACT FROM AUTHOR]