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Additional file 2.

(A) Detailed 2-DE images of Annexin A2 protein spots in A549/DDP cells compared with A549 cells. (B) Mascot database search based on MALDI-TOF-MS/MS matched to Annexin A2 with highly significant scores. (C) Peptide information for Annexin A2 based on MALDI-TOF-MS/MS analysis. (D) MS/MS Fragmentation of sequence ‘GVDEVTIVNILTNR’. (E) The amino acid sequences of Annexin A2, in which matched peptide sequences are underlined. Figure S2. Cells were treated with cisplatin at the indicated concentration for 48 h, and cell viability was measured by MTT assay. Table indicates the IC50 values for each cell. Figure S3. (A) H460 and H1650 cells transfected with pCMV6-Annexin A2 were treated with cisplatin at the indicated concentration for 48 h, and cell viability was measured by MTT assay. Table indicates the IC50 values for each condition. (B) H460 and H1650 cells transfected with pCMV6-Annexin A2 were treated with cisplatin at the indicated concentration for 14 days, (Left) Colonies were fixed with acetic acid-methanol (1:4) and stained with crystal violet. (Right) The number of colonies was from three independent experiments. *P

List of primary antibodies used in the study. (DOCX 17 kb)