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Additional file 7: Supplementary Table S3. Primers used for qRT-PCR in this study.

Additional file 14: Supplementary Table S10. Bioinformatics analysis of TCONS_00054154

Additional file 9: Supplementary Table S5. LD decay.

In this paper, response surface methodology is used to study alkali-slag concrete(ASC) fracture toughness; the influence on fracture toughness from high to low is slag content, age and sol ratio, the interaction of slag content and age is the most significant; initiation fracture toughness is about 50%~70% of unstable fracture toughness, crack initiation should be the warning indicator of ASC structure; fracture toughness decreases with freeze-thaw times increasing, its declining rate and degree increase with sol ratio and slag content; increase of sol ratio reduces internal density and hinders depolymerization. Cementitious material content reduces ASC toughness.