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1. Additional file 13: of Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes

Author

Attardo, Geoffrey, Adly Abd-Alla, Acosta-Serrano, Alvaro, Allen, James, Bateta, Rosemary, Benoit, Joshua, Bourtzis, Kostas, Caers, Jelle, Caljon, Guy, Christensen, Mikkel, Farrow, David, Friedrich, Markus, Aurélie Hua-Van, Jennings, Emily, Larkin, Denis, Lawson, Daniel, Lehane, Michael, Lenis, Vasileios, Lowy-Gallego, Ernesto, Macharia, Rosaline, Malacrida, Anna, Marco, Heather, Masiga, Daniel, Maslen, Gareth, Matetovici, Irina, Meisel, Richard, Meki, Irene, Michalkova, Veronika, Miller, Wolfgang, Minx, Patrick, Mireji, Paul, Ometto, Lino, Parker, Andrew, Rio, Rita, Rose, Clair, Rosendale, Andrew, Rota-Stabelli, Omar, Savini, Grazia, Schoofs, Liliane, Scolari, Francesca, Swain, Martin, Takáč, Peter, Tomlinson, Chad, Tsiamis, George, Abbeele, Jan, Vigneron, Aurelien, Jingwen Wang, Warren, Wesley, Waterhouse, Robert, Weirauch, Matthew, Weiss, Brian, Wilson, Richard, Zhao, Xin, and Aksoy, Serap

Abstract

Review history. (DOCX 31 kb)

Published
2019
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2. Additional file 1: of Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes

Author

Attardo, Geoffrey, Adly Abd-Alla, Acosta-Serrano, Alvaro, Allen, James, Bateta, Rosemary, Benoit, Joshua, Bourtzis, Kostas, Caers, Jelle, Caljon, Guy, Christensen, Mikkel, Farrow, David, Friedrich, Markus, Aurélie Hua-Van, Jennings, Emily, Larkin, Denis, Lawson, Daniel, Lehane, Michael, Lenis, Vasileios, Lowy-Gallego, Ernesto, Macharia, Rosaline, Malacrida, Anna, Marco, Heather, Masiga, Daniel, Maslen, Gareth, Matetovici, Irina, Meisel, Richard, Meki, Irene, Michalkova, Veronika, Miller, Wolfgang, Minx, Patrick, Mireji, Paul, Ometto, Lino, Parker, Andrew, Rio, Rita, Rose, Clair, Rosendale, Andrew, Rota-Stabelli, Omar, Savini, Grazia, Schoofs, Liliane, Scolari, Francesca, Swain, Martin, Takáč, Peter, Tomlinson, Chad, Tsiamis, George, Abbeele, Jan, Vigneron, Aurelien, Jingwen Wang, Warren, Wesley, Waterhouse, Robert, Weirauch, Matthew, Weiss, Brian, Wilson, Richard, Zhao, Xin, and Aksoy, Serap

Abstract

Supplementary text and supplementary tables. (DOCX 186 kb)

Published
2019
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4. Additional file 3: of Effect of antibiotic treatment and gamma-irradiation on cuticular hydrocarbon profiles and mate choice in tsetse flies (Glossina m. morsitans)

Author

Engl, Tobias, Michalkova, Veronika, Weiss, Brian, GĂźler Uzel, Takac, Peter, Miller, Wolfgang, Adly Abd-Alla, Aksoy, Serap, and Kaltenpoth, Martin

Subjects
fungi
Abstract

Effect of the time point of gamma-irradiation on CHC profiles of individually reared 10Â day old adult G. m. morsitans males. Discriminant analysis based on log-ratio transformed relative amounts across irradiation time points (early and late pupal development and as young adults). (PDF 1354Â kb)

Published
2018
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5. Additional file 1: of Effect of antibiotic treatment and gamma-irradiation on cuticular hydrocarbon profiles and mate choice in tsetse flies (Glossina m. morsitans)

Author

Engl, Tobias, Michalkova, Veronika, Weiss, Brian, GĂźler Uzel, Takac, Peter, Miller, Wolfgang, Adly Abd-Alla, Aksoy, Serap, and Kaltenpoth, Martin

Subjects
fungi
Abstract

Comparison of CHC profiles of untreated (Ctr) and tetracycline-treated (Tet) (a) female and (b) male tsetse flies (G. m. morsitans) across the three different experiments. (PDF 1363Â kb)

Published
2018
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6. Additional file 4: of Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans

Author

Scolari, Francesca, Attardo, Geoffrey, Aksoy, Emre, Weiss, Brian, Savini, Grazia, Takac, Peter, Adly Abd-Alla, Parker, Andrew, Aksoy, Serap, and Malacrida, Anna

Abstract

Gene Ontology analysis of genes down-regulated in GmmApo male reproductive tissues. The number of genes associated with corresponding Gene Ontology terms (Biological Process, Molecular Function, and Cellular Component Level III) is shown. (PDF 784Â kb)

Published
2018
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7. Additional file 3: of Effect of antibiotic treatment and gamma-irradiation on cuticular hydrocarbon profiles and mate choice in tsetse flies (Glossina m. morsitans)

Author

Engl, Tobias, Michalkova, Veronika, Weiss, Brian, GĂźler Uzel, Takac, Peter, Miller, Wolfgang, Adly Abd-Alla, Aksoy, Serap, and Kaltenpoth, Martin

Subjects
fungi
Abstract

Effect of the time point of gamma-irradiation on CHC profiles of individually reared 10Â day old adult G. m. morsitans males. Discriminant analysis based on log-ratio transformed relative amounts across irradiation time points (early and late pupal development and as young adults). (PDF 1354Â kb)

Published
2018
Full Text
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8. Additional file 7: of Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans

Author

Scolari, Francesca, Attardo, Geoffrey, Aksoy, Emre, Weiss, Brian, Savini, Grazia, Takac, Peter, Adly Abd-Alla, Parker, Andrew, Aksoy, Serap, and Malacrida, Anna

Subjects
nervous system, sense organs, skin and connective tissue diseases
Abstract

Changes in transcript abundance in response to maturation and mating in the presence of symbionts of genes down- and up-regulated in GmmApo male reproductive tissues. Fold change difference in expression is separately shown for MAG- and testes-biased genes for A) GmmApo down-regulated, and B) GmmApo up-regulated genes. (PDF 893Â kb)

Published
2018
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11. Additional file 1: of Analysis of the gut-specific microbiome from field-captured tsetse flies, and its potential relevance to host trypanosome vector competence

Author

Griffith, Bridget, Weiss, Brian, Aksoy, Emre, Mireji, Paul, Auma, Joana, Wamwiri, Florence, Echodu, Richard, Murilla, Grace, and Aksoy, Serap

Subjects
fungi, parasitic diseases
Abstract

Table S1. Bacteria isolated from tsetse fly midguts using culture dependent techniques. Table S2. PCR primers used in this study. Figure S1. Average taxonomic composition and % abundance of environmentally acquired (top graph) and symbiotic bacteria (bottom graph) found in midguts of uninfected and trypanosome infected G. pallidipes collected in Nguruman, Kenya, Shimba Hills, Kenya and Murchison Falls, Uganda. (DOCX 508 kb)

Published
2018
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12. Additional file 5: of Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans

Author

Scolari, Francesca, Attardo, Geoffrey, Aksoy, Emre, Weiss, Brian, Savini, Grazia, Takac, Peter, Adly Abd-Alla, Parker, Andrew, Aksoy, Serap, and Malacrida, Anna

Abstract

Gene Ontology analysis of genes up-regulated in GmmApo male reproductive tissues. The number of genes associated with corresponding Gene Ontology terms (Biological Process, Molecular Function, and Cellular Component Level III) is shown. (PDF 778Â kb)

Published
2018
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13. Additional file 2: of Effect of antibiotic treatment and gamma-irradiation on cuticular hydrocarbon profiles and mate choice in tsetse flies (Glossina m. morsitans)

Author

Engl, Tobias, Michalkova, Veronika, Weiss, Brian, GĂźler Uzel, Takac, Peter, Miller, Wolfgang, Adly Abd-Alla, Aksoy, Serap, and Kaltenpoth, Martin

Abstract

Effect of gamma-irradiation dose and time point on CHC profiles of 10Â day old individually reared adult G. m. morsitans males. Discriminant analysis based on log-ratio transformed relative amounts across all treatment groups (time points and irradiation doses). (PDF 1353Â kb)

Published
2018
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14. Additional file 2: Table S2. of Tsetse fly (Glossina pallidipes) midgut responses to Trypanosoma brucei challenge

Author

Bateta, Rosemary, Jingwen Wang, Yineng Wu, Weiss, Brian, Warren, Wesley, Murilla, Grace, Aksoy, Serap, and Mireji, Paul

Subjects
genetic processes, natural sciences
Abstract

Validation of G. pallidipes RNA Seq data with qPCR RNA-seq expression values (log2 ratios) for ten genes plotted against qPCR values (log2 ratios). Figure S1. RNA-seq expression values (log2 ratios) for ten genes plotted against qPCR values (log2 ratios). (PDF 1742Â kb)

Published
2017
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15. Additional file 2: Table S2. of Tsetse fly (Glossina pallidipes) midgut responses to Trypanosoma brucei challenge

Author

Bateta, Rosemary, Jingwen Wang, Yineng Wu, Weiss, Brian, Warren, Wesley, Murilla, Grace, Aksoy, Serap, and Mireji, Paul

Subjects
genetic processes, natural sciences
Abstract

Validation of G. pallidipes RNA Seq data with qPCR RNA-seq expression values (log2 ratios) for ten genes plotted against qPCR values (log2 ratios). Figure S1. RNA-seq expression values (log2 ratios) for ten genes plotted against qPCR values (log2 ratios). (PDF 1742Â kb)

Published
2017
Full Text
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18. Additional file 1: of Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans

Author

Scolari, Francesca, Attardo, Geoffrey, Aksoy, Emre, Weiss, Brian, Savini, Grazia, Takac, Peter, Adly Abd-Alla, Parker, Andrew, Aksoy, Serap, and Malacrida, Anna

Subjects
fungi, bacteria, 3. Good health
Abstract

The effect of tetracycline treatment on the maternal transmission of tsetse endosymbionts. PCR assays on genomic DNA showing that GmmWT flies were positive for Wigglesworthia (Wig Thic), Sodalis (Sod SsaC) and Wolbachia (Wol GroEL), whereas offspring resulting from tetracycline-treated flies lack all three symbionts. The bottom panel shows the amplification of tsetse tubulin beta-1 gene on all samples. A and B lanes represent two PCR replicates performed on pooled genomic DNA from ten males and ten females extracted either at 2 and 8 days post-emergence. (PDF 1070Â kb)

19. Additional file 1: of Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans

Author

Scolari, Francesca, Attardo, Geoffrey, Aksoy, Emre, Weiss, Brian, Savini, Grazia, Takac, Peter, Adly Abd-Alla, Parker, Andrew, Aksoy, Serap, and Malacrida, Anna

Subjects
fungi, bacteria, 3. Good health
Abstract

The effect of tetracycline treatment on the maternal transmission of tsetse endosymbionts. PCR assays on genomic DNA showing that GmmWT flies were positive for Wigglesworthia (Wig Thic), Sodalis (Sod SsaC) and Wolbachia (Wol GroEL), whereas offspring resulting from tetracycline-treated flies lack all three symbionts. The bottom panel shows the amplification of tsetse tubulin beta-1 gene on all samples. A and B lanes represent two PCR replicates performed on pooled genomic DNA from ten males and ten females extracted either at 2 and 8 days post-emergence. (PDF 1070Â kb)

20. Additional file 2: of Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes

Author

Attardo, Geoffrey, Adly Abd-Alla, Acosta-Serrano, Alvaro, Allen, James, Bateta, Rosemary, Benoit, Joshua, Bourtzis, Kostas, Caers, Jelle, Caljon, Guy, Christensen, Mikkel, Farrow, David, Friedrich, Markus, Aurélie Hua-Van, Jennings, Emily, Larkin, Denis, Lawson, Daniel, Lehane, Michael, Lenis, Vasileios, Lowy-Gallego, Ernesto, Macharia, Rosaline, Malacrida, Anna, Marco, Heather, Masiga, Daniel, Maslen, Gareth, Matetovici, Irina, Meisel, Richard, Meki, Irene, Michalkova, Veronika, Miller, Wolfgang, Minx, Patrick, Mireji, Paul, Ometto, Lino, Parker, Andrew, Rio, Rita, Rose, Clair, Rosendale, Andrew, Rota-Stabelli, Omar, Savini, Grazia, Schoofs, Liliane, Scolari, Francesca, Swain, Martin, Takáč, Peter, Tomlinson, Chad, Tsiamis, George, Abbeele, Jan, Vigneron, Aurelien, Jingwen Wang, Warren, Wesley, Waterhouse, Robert, Weirauch, Matthew, Weiss, Brian, Wilson, Richard, Zhao, Xin, and Aksoy, Serap

Subjects
3. Good health
Abstract

Supplemental Figures S1-S12 and associated captions. (PDF 2260 kb)

21. Additional file 2: of Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes

Author

Attardo, Geoffrey, Adly Abd-Alla, Acosta-Serrano, Alvaro, Allen, James, Bateta, Rosemary, Benoit, Joshua, Bourtzis, Kostas, Caers, Jelle, Caljon, Guy, Christensen, Mikkel, Farrow, David, Friedrich, Markus, Aurélie Hua-Van, Jennings, Emily, Larkin, Denis, Lawson, Daniel, Lehane, Michael, Lenis, Vasileios, Lowy-Gallego, Ernesto, Macharia, Rosaline, Malacrida, Anna, Marco, Heather, Masiga, Daniel, Maslen, Gareth, Matetovici, Irina, Meisel, Richard, Meki, Irene, Michalkova, Veronika, Miller, Wolfgang, Minx, Patrick, Mireji, Paul, Ometto, Lino, Parker, Andrew, Rio, Rita, Rose, Clair, Rosendale, Andrew, Rota-Stabelli, Omar, Savini, Grazia, Schoofs, Liliane, Scolari, Francesca, Swain, Martin, Takáč, Peter, Tomlinson, Chad, Tsiamis, George, Abbeele, Jan, Vigneron, Aurelien, Jingwen Wang, Warren, Wesley, Waterhouse, Robert, Weirauch, Matthew, Weiss, Brian, Wilson, Richard, Zhao, Xin, and Aksoy, Serap

Subjects
3. Good health
Abstract

Supplemental Figures S1-S12 and associated captions. (PDF 2260 kb)

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