Search

Western blotting of adult female ticks during blood-feeding. Protein lysates were extracted from adult female ticks during the different stages of blood-feeding. Protein lysates were run on 12% SDS-PAGE gel before being transferred to PVDF membranes and subjected to Western blotting. Mouse tubulin antiserum was used as control. Leftmost lane indicates markers for molecular weight. (PDF 253 kb)

The modeled tertiary structures of HlGST (a) and HlGST2 (b). The model is based on template c1b8xA [48] constructed using PHYRE2 software [49]. Green indicates the N-terminal domain containing the GSH binding site (orange), while red indicates the C-terminal domain containing the substrate binding site (blue). The mu-loop is indicated by bluish green. (PDF 193 kb)

P- and t-values of GST transcription profiles. (DOCX 40 kb)

RT-PCR (a) and Western blotting (b) of knockdown ticks. Ticks were silenced by injecting 1 Îźg of double-stranded RNA (dsRNA) per tick. a Total RNA was extracted from whole 4-day fed GST and EGFP knockdown ticks. cDNA was synthesized and subjected to RT-PCR. PCR products were run on 1.5% TAE agarose gel and stained with ethidium bromide. Actin was used as loading control. b Protein lysates were extracted from whole 4-day-fed HlGST, HlGST2, HlGST1/2, and EGFP knockdown ticks. Protein lysates were run on 12% SDS-PAGE gel before being transferred to polyvinylidene difluoride (PVDF) membranes and subjected to Western blotting. Mouse tubulin antiserum was used as control. (PDF 200 kb)

Nucleotide and deduced amino acid sequences of HlGST (a) and HlGST2 (b) of Haemaphysalis longicornis. Start and stop codons are underlined. Predicted glutathione and substrate binding sites are shaded in black and gray, respectively. The putative polyadenylation signal, AATAAA, is double underlined. (PDF 53 kb)

Effect of HlGST and/or HlGST2 knockdown on ticks. (DOCX 36 kb)

Effect of HlGST and/or HlGST2 knockdown on ticks. (DOCX 36 kb)

P- and t-values of GST transcription profiles. (DOCX 40 kb)