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Additional file 1: Figure S1. Immunoblot analysis visualizing the expression level of MOA in leave (L) and root (R) crude extracts. The blot was exposed in different timepoints: 1s (A), 10s (B) and 20s (C). FLAG-tagged proteins were detected with anti-FLAG antibodies. (D) Ponceau-S stained immunoblot was used as a blotting control. (E) Coomassie brilliant blue stained SDS-PAGE was used as a loading control. Molecular weights of marker proteins (M) are indicated.

Additional file 3: Figure S2. Plant colonization effects on soil microbial properties. Mean values with confidence intervals of basal respiration [µg O2 g−1 soil dry mass h−1] (a) two and (c) five years, and soil microbial biomass [µg C g−1 soil dry mass] (b) two and (d) five years after colonization by plant species. Circles display basal respiration and soil microbial biomass with resident plant species of weeded subplots, respectively, and open circles display basal respiration and soil microbial biomass with resident plant species plus colonizing plant species of non-weeded subplots, respectively.

Additional file 2: Figure S1. Plant colonization effects on plant cover and plant biomass. Mean values with confidence intervals of colonizing plant species after (a) two years and (b) five years. (c) Mean values with confidence intervals of plant cover [%] after two years and (d) plant biomass [g mâ 2] after five years. In c) and d) circles display plant cover and biomass of resident plant species of weeded subplots, respectively, and open circles display plant cover and biomass with resident plant species plus colonizing plant species of non-weeded subplots, respectively.