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Additional file 1: Supplementary file 1 A-M. The evaluation of cytopathic effects in SHK-1 cells infected with P. salmonis. Separate images of cell cultures under four experimental conditions at days 4, 7 and 11 (dpi): non-infected SHK-1 not treated with DFO (SHK-1), non-infected SHK-1 cells treated with DFO (SHK-1 + DFO), infected SHK-1 cells not treated with DFO (SHK-1 + P. sal) and SHK-1 cells infected with P. salmonis and treated with DFO (SHK-1 + P. sal + DFO). Images were taken with the EVOS® FL Color Imaging System (Invitrogen, Thermo Fisher Scientific Inc., Carlsbad, CA, USA) using 10X objective after 3 PBS-1X washes. The scale bar was added by using the image analysis ImageJ 1.37 software (National Institutes of Health, Bethesda, MD, USA). Scale bar = 260 μM.

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Additional file of Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse (Lepeophtheirus salmonis) revealed bi-phasic responses coinciding with the copepod-chalimus transition

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Table S1. Primers used for real-time qPCR analysis. Table S2. Formulation and proximate compositions of the feeds. (DOCX 23Â kb)

An overview of samples and analysis methods applied in Trial 1, 2 and 3. Table S2. Scoring of liver steatosis in individual sections based on [88]. Table S3. Primer list. (DOCX 20 kb)