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1. Additional file 5 of Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA

Author

Deleu, Jill, Schoofs, Kathleen, Decock, Anneleen, Verniers, Kimberly, Roelandt, Sofie, Denolf, Angie, Verreth, Joke, De Wilde, Bram, Van Maerken, Tom, De Preter, Katleen, and Vandesompele, Jo

Abstract

Additional file 5: Figure S2. Overview of TapeStation results. Results from TapeStation of all cfDNA samples purified with one of the six (co-)purification kits. There are 13/46 samples that have concentrations below LOD (20 pg/µl), and 2/46 samples without any peaks (flat profiles). For 1/46 samples there was not enough material left for TapeStation.

Published
2023
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2. Additional file 6 of Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA

Author

Deleu, Jill, Schoofs, Kathleen, Decock, Anneleen, Verniers, Kimberly, Roelandt, Sofie, Denolf, Angie, Verreth, Joke, De Wilde, Bram, Van Maerken, Tom, De Preter, Katleen, and Vandesompele, Jo

Abstract

Additional file 6: Figure S3. Detailed description of laboratory workflow. Workflow used to determine co-purification performance of each kit and subsequent calculations to determine the concentrations in each eluate: (A) MIRA and CCF, (B) MIR, (C) CAT, (D) MAX, and (E) MAP.

Published
2023
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3. Additional file 7 of Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA

Author

Deleu, Jill, Schoofs, Kathleen, Decock, Anneleen, Verniers, Kimberly, Roelandt, Sofie, Denolf, Angie, Verreth, Joke, De Wilde, Bram, Van Maerken, Tom, De Preter, Katleen, and Vandesompele, Jo

Abstract

Additional file 7: Figure S4. Experimental overview of a combined cfDNA/cfRNA quantification and a parallel quantification of cfDNA and cfRNA.

Published
2023
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