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Additional file 4: Figure S1. Activity of Rm1a and Rm4a. Figure S2. Cytotoxicity of R. metallica aculeatoxin peptides. Figure S3. Gene structures of some ant aculeatoxins. Figure S4. Presence of aculeatoxin alleles among workers from the same R. metallica colony. Figure S5. Phylogeny of R. metallica aculeatoxins estimated from full-length or only signal- and propeptide domains. Table S4. Recombination analysis of R. metallica clades 1–3.Table S5. Primers used to amplify aculeatoxins from R. metallica genomic DNA.

Additional file 2: Table S1. Peptides and proteins identified in the pooled venom of R. metallica. Peptide and protein names and corresponding encoding contig id are provided for each, along with the best BLAST hit against UniProtKB, evidence supporting their presence in the venom, mature molecular weight, expression level (TPM), and the sequence of the mature domain and full prepropeptide. Complete (1) evidence includes identification of the complete mature peptide sequence from either the bottom-up or top-down proteomic experiments, while “partial” includes only partial identification from the bottom-up experiment.