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Additional file 6. Table S2: Predicted ORFs and genes encoded by the UPWr_S phage genomes. Protein sequences of the predicted ORFs of UPWr_S phages were subjected to the BLASTp program to analyze their best known matches on the NCBI website ( https://blast.ncbi.nlm.nih.gov ). The average nucleotide identity and query coverage were calculated by BLASTp with the cutoff E-value set at 1E-04. “Related phages” refers to a top hit from NCBI BLASTp.

Additional file 4. Fig. S2: One-step growth curves of UPWr_S phages. The one-step growth curve of UPWr_S phages propagated on their respective hosts in LB medium revealed that the latent periods and burst sizes were approximately 15, 12, 9, 24, 23 and 92 minutes for phages (a) UPWr_S1, (b) UPWr_S2, (c) UPWr_S3, (d) UPWr_S4 and (e) UPWr_S5, respectively. The average burst size was estimated to be 201, 89, 92, 48, 92 PFU/cell for phages UPWr_S1, UPWr_S2, UPWr_S3, UPWr_S4 and UPWr_S5, respectively.

Additional file 1: File S1. R code to analyse and plot UPWr_S phages host ranges and EOP and construct a heatmap. For host range determination 64 Salmonella strains belonging to 10 serovars were tested. UPWr_S phages showed a high ability to infect the majority of tested Salmonella strains belonging to Enteritidis and Gallinarum serovars, and all of them infected S. Senftenberg. Only the phages UPWr_S2 and UPWr_S3 could lyse the majority of strains belonging to S. Typhimurium nonspecifically, utilizing the lysis from without mechanism, and S. Stanley. S. Chester strains were lysed only by UPWr_S3.

Additional file 2. Table S1. List of phages used in a comparison study to construct the phylogenetic tree in Fig. 4 and their order in the phylogenetic tree. For evolutionary relationships, 60 available genomes of bacteriophages belonging to Jerseyvirus and six available genomes of Cornellvirus bacteriophages, both members of the Guernseyvirinae subfamily, Siphoviridae family, and 29 representative genomes from other phage genera infecting Salmonella, were selected from the GenBank Virus database.