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Additional file 12. Comparison of full-length amino acid sequences of 11 genomic segments of PRG942V-80 (G9P[23]) vaccine strain with its different passages. The full-length amino acid sequences of the 11 genomic segments of the 80th-passage attenuated PRG942V-80 vaccine strain was compared with those of the 20th-, 40th-, and 60th-passage attenuated strains and the original virulent strain.

Additional file 14. Summary of the histopathological findings in the small intestine of the colostrums-deprived neonatal piglets vaccinated with a live attenuated monovalent vaccine strain (174-1V-80) or filtered supernatant of a mixture of homogenized small intestine and feces sampled from each passage.

Additional file 2. Oligonucleotide primers for sequencing or for 5′ and 3′ RACE PCRs of all eleven genomic segments of the porcine 174-1, PRG942, and K71 strains and their passages. Listed in the table are the primer pairs used to generate the full-length sequence of Korean porcine 174-1, PRG942, and K71 rotavirus strains. Also indicated are the gene-specific primers used for 5′ and 3′ RACE PCR.

Additional file 6. Virus titers of each strain in different passages.

Additional file 15. Summary of the histopathological findings in the small intestine of the colostrums-deprived neonatal piglets vaccinated with a live attenuated monovalent vaccine strain (PRG942V-80) or filtered supernatant of a mixture of homogenized small intestine and feces sampled from each passage.

Additional file 16. Summary of the histopathological findings in the small intestine of the colostrums-deprived neonatal piglets vaccinated with a live attenuated monovalent vaccine strain (K71V-80) or filtered supernatant of a mixture of homogenized small intestine and feces sampled from each passage.

Additional file 1. Comparison of genotype constellation of porcine 174-1 (G8P[7]), PRG942 (G9P[23]), and K71 (G5P[7]) strains with other known reference genotypes.

Additional file 11. Comparison of full-length amino acid sequences of 11 genomic segments of 174-1V-80 (G8P[7]) vaccine strain with its different passages. The full-length amino acid sequences of the 11 genomic segments of the 80th-passage attenuated 174-1V-80 vaccine strain was compared with those of the 20th-, 40th-, and 60th-passage attenuated strains and the original virulent strain.

Additional file 5. Summary of safety test results for a live attenuated porcine rotavirus monovalent vaccine strains 174-1V-80, PRG942V-80, and K71V-80 in mice, guinea pigs, and pigs.

Additional file 4. Summary of the histopathological findings in the small intestine of the colostrums-deprived neonatal piglets inoculated with each virulent strains (174-1, PRG942, and K71), or immunized with each live attenuated monovalent or trivalent vaccines and then challenged with each corresponding original virulent strain(s).

Additional file 3. Full-length ORF nucleotide sequence identities (%) of ORFa in 11 genomic segments between three Korean porcine 174-1, PRG942, and K71 strains and other known strains representative of corresponding or neighbor genotypes. The nucleotide sequences of open reading frame of the virulent and attenuated porcine 174-1, PRG942, and K71 rotavirus strains were compared with known RVA strains. The values represent the nucleotide similarity of porcine 174-1, PRG942, and K71with the reference strains.

Additional file 13. Comparison of full-length amino acid sequences of 11 genomic segments of K71V-80 (G9P[23]) vaccine strain with its different passages. The full-length amino acid sequences of the 11 genomic segments of the 80th-passage attenuated K71V-80 vaccine strain was compared with those of the 20th-, 40th-, and 60th-passage attenuated strains and the original virulent strain.

Additional file 1. Sequences of siRNAs against target molecules and scrambled siRNA used in this study.