Search

Three-dimensional PCA score scatter plots. Three-dimensional PCA score scatter plots obtained for 37 septic shock patients (red) and 20 ICU controls (green) based on the (A) metabolic data and (B) cytokine/chemokine data. The groups are well clustered along the axes of the three principal components. One septic shock sample is placed outside the sphere that describes the 95% confidence interval of the Hotelling’s T-squared distribution.

The receiver operating characteristic (ROC) curves plotted with a decimal logarithmic scale for the horizontal axes. The ROC plots for (A) septic shock patients vs. ICU controls and (B) septic shock nonsurvivors vs. septic shock survivors models based on the metabolomics data, cytokine/chemokine data and the combined dataset (metabolites together with inflammatory mediators), APACHE (acute physiology and chronic health evaluation) and SOFA (sequential organ failure assessment) scores. Black line - fit line, red dashed line - the chance curve (that is the diagonal of the ROC curve when plotted in linear scale).

Clinical mechanism. (DOCX 19 kb)

Preclinical intervention. (DOCX 36 kb)

Supplemental descriptions of methods, statistical modeling, results of subgroups, discussion of the importance of the metabolites and protein mediators of interest (biomarkers) identified, and supplemental references. This file also includes two tables. Table E1. The metabolites (non-targeted 1H NMR spectroscopy and metabolite concentration profiling) and protein mediators (targeted bead-based multiplex assay) detected and quantified. Table E2. Comparison of statistical measures calculated for the supervised OPLS-DA models without excluding outliers from the results presented in the main text of the article. (PDF 307 kb)

Best-evidence synthesis. (DOCX 15 kb)

Search strategies. (DOCX 17 kb)

The OPLS-DA regression coefficient plot. Positive values of coefficients (the upper part of the diagram) indicates increased metabolite or inflammatory mediator concentrations in septic shock nonsurvivor samples while negative values (the lower part of diagram) present a decrease in metabolite or inflammatory mediator concentrations, as compared to the age-sex-matched septic shock survivors. Only significant metabolites are shown (P

Clinical intervention. (DOCX 22 kb)

Clinical biomarkers. (DOCX 76 kb)

The receiver operating characteristic (ROC) curves plotted with a decimal logarithmic scale for the horizontal axes. The ROC plots for (A) septic shock patients vs. ICU controls and (B) septic shock nonsurvivors vs. septic shock survivors models based on the metabolomics data, cytokine/chemokine data and the combined dataset (metabolites together with inflammatory mediators), APACHE (acute physiology and chronic health evaluation) and SOFA (sequential organ failure assessment) scores. Black line - fit line, red dashed line - the chance curve (that is the diagonal of the ROC curve when plotted in linear scale).

The summary table. Comparison of statistical measures calculated for the supervised OPLS-DA models without excluding outliers to the results presented in the manuscript.