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Length and number of sequences for branch differentially expressed genes. (PDF 125Â kb)

Melting curves and efficiencies of primers for quantitative real- time PCR. (PDF 192Â kb)

Significantly differentially expressed transcripts plot for stem-branch genes. Red plots indicate transcripts differentially expressed and black spots transcripts expressed in common. (PDF 144Â kb)

Relevant enzymes found for differentially expressed genes in stem. In Blue, sequences higher than 3000Â bp. (PDF 114Â kb)

43 genes highly differentially expressed between stem secondary xylem from 12- and 60-year-old trees. (PDF 106Â kb)

Other relevant differentially expressed genes from secondary xylem. We chose other genes with the highest expression between young (12-years-old) and mature (60-years-old) trees, and performed a transformation of root square in order to visualize their values. (PDF 178Â kb)

GO frequencies for differentially expressed (DE) transcripts. Stem and Branch secondary xylem were the tissues presented in the table. DE transcripts were obtained when comparing 12- and 60-year-old teak trees in both tissues. The first column for GO frequencies is organized from lowest to highest. (PDF 119Â kb)

Gene ontology (GO) assignment for the unigenes differentially expressed of T. grandis branch secondary xylem. GO assignments (multilevel pie chart with term filter value 5) as predicted for (a) biological process, (b) molecular function and (c) cellular components. The number of unigenes assigned to each GO term is shown behind semicolon. (PDF 143Â kb)

RIN factor of all samples used for Illumina sequencing. (PDF 225Â kb)

Primers for quantitative real-time PCR. (PDF 90Â kb)

Branch secondary xylem pathways found by Kegg. (PDF 116Â kb)

Differential expression of log2 ratio (fold change) versus mean between different conditions with DESeq program. a) Dispersion plot for branch secondary xylem transcripts. b) Significantly differentially expressed transcripts scatterplot for branch secondary xylem transcripts. c) Dispersion plot for stem secondary xylem transcripts. d) Significantly differentially expressed transcripts scatterplot for stem secondary xylem transcripts. e) Significantly differentially expressed transcripts scatterplot for branch secondary xylem against flower, seedling, leaf and root. f) Significantly differentially expressed transcripts scatterplot for stem secondary xylem against flower, seedling, leaf and root. Fitted curve of the spots is in red. Red dots indicate transcripts differentially expressed at 10 % false discovery rate and black spots transcripts are expressed in common [61]. (PDF 132 kb)

Predicted MYB domain protein sequences from Tectona grandis . Amino acid sequences of the four MYB transcription factors were obtained with ExPASy Translate tool ( http://web.expasy.org/translate/ ). Grey shading indicates identical amino acid residues that agree with the motifs referenced by Bedon et al. (2007). MYB-CC type transfactor domain (TgMYB1) and R2R-MYB DNA-binding domains (MYBR2R3-DBDs) (TgMYB2, TgMYB3, TgMYB4) are indicated. bHLH motif ([DE]L × 2 [RK] × 3 L × 6 L × 3R) is indicated in TgMYB3. (PDF 131 kb)

Stem secondary xylem pathways found by Kegg. (PDF 129Â kb)

Raw data, cleaning data and assembly. (PDF 93Â kb)

FASTQC reports of each sample from the RNAseq of Tectona grandis. (PDF 2000Â kb)

Some transcripts obtained from RNA-seq in Tectona grandis and used for subsequent analysis. Four MYB transcription factors: TgMYB1 (NCBI Accession number KR092428), TgMYB2 (NCBI Accession number KR092429), TgMYB3 (NCBI Accession number KR092430), TgMYB4 (NCBI Accession number KR092431), three heat-shock proteins: TgHsp1 (NCBI Accession number KR092432), TgHsp2 (NCBI Accession number KR092433), TgHsp3 (NCBI Accession number KR092434), carboxylesterase: TgCES (NCBI Accession number KR092436), bax inhibitor: TgBi (NCBI Accession number KR092435). In yellow, the methionine. In green, the stop codon. In grey, the coding sequence. In blue, the real-time PCR primers. (PDF 125Â kb)