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Figure S1. Boxplot summarising sequencing statistics including total purity-filtered reads, uniquely aligned reads, multimapping reads, unaligned reads and reads that were removed due to a very short insert size (

Representative cytograms of cell surface markers CD44 and CD24 obtained using flow cytometry. JIMT-1 cells were treated with 50 nM salinomycin or salinomycin analogs for 72 h. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester. (DOCX 1340 kb)

Representative cytograms of cell surface markers CD44 and CD24 obtained using flow cytometry. JIMT-1 cells were treated with salinomycin or salinomycin analogs at IC50 for 72 h. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester. (DOCX 1331 kb)

Representative cytograms of ALDH assay obtained using flow cytometry. JIMT-1 cells were treated with salinomycin or salinomycin analogs at IC50 for 72 h. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester. (DOCX 1597 kb)

Representative cytograms of ALDH assay obtained using flow cytometry. JIMT-1 cells were treated with 50 nM salinomycin or salinomycin analogs for 72 h. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester. (DOCX 1678 kb)

Representative cytograms of ALDH assay obtained using flow cytometry. JIMT-1 cells were treated with salinomycin or salinomycin analogs at IC50 for 72 h. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester. (DOCX 1597 kb)

Salinomycin treatment decreases the proportion of ALDH+ in a dose dependent manner. JIMT-1 cells were treated with salinomycin for 72 h at the indicated concentrations. The effect on the ALDH+ population was determined using flow cytometry. Data are represented as mean ± SEM for n = 3. (DOCX 38 kb)

U-shaped dose response curve found for the CD44+ /CD24− population in N–ethyl carbamate 2a-treated JIMT-1 cells. JIMT-1 cells were treated with 2a for 72 h at the indicated concentrations. The effect on the CD44+/CD24− population was determined using flow cytometry. Data are represented as mean ± SEM for n = 4. (DOCX 41 kb)

Salinomycin treatment decreases the proportion of ALDH+ in a dose dependent manner. JIMT-1 cells were treated with salinomycin for 72 h at the indicated concentrations. The effect on the ALDH+ population was determined using flow cytometry. Data are represented as mean ± SEM for n = 3. (DOCX 38 kb)

Representative cytograms of ALDH assay obtained using flow cytometry. JIMT-1 cells were treated with 50 nM salinomycin or salinomycin analogs for 72 h. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester. (DOCX 1678 kb)

Representative cytograms of cell surface markers CD44 and CD24 obtained using flow cytometry. JIMT-1 cells were treated with salinomycin or salinomycin analogs at IC50 for 72 h. SA: salinomycin, 2a: carbamate, 2b: acetate, 2c: carbonate and 3: C1-methyl ester. (DOCX 1331 kb)

U-shaped dose response curve found for the CD44+ /CD24− population in N–ethyl carbamate 2a-treated JIMT-1 cells. JIMT-1 cells were treated with 2a for 72 h at the indicated concentrations. The effect on the CD44+/CD24− population was determined using flow cytometry. Data are represented as mean ± SEM for n = 4. (DOCX 41 kb)