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Additional file 2. Supplementary materials and methods.

Additional file 3 Figure S1. The expression and correlation analysis between ZFAS1 and snoRNP complex based on the TCGA database. Figure S2. The (m) RNA expression of ZFAS1, NOP58, SNORD12C, and SNORD78 in CRC tissues and matched tumor-adjacent controls (n = 30). Figure S3. The correlation analysis of ZFAS1, NOP58, SNORD12C/78 expression in 30 paired CRC and control tissues. Figure S4. The effect of ZFAS1 on cell proliferation and apoptosis in CRC cells. Figure S5. The correlation of ZFAS1 and NOP58 expression and prognosis evaluation in CRC patient tissues. Figure S6. The 2′-O-Me activity levels after interfering ZFAS1 and/or NOP58 expression. Figure S7. The 2′-O-Me activities mediated by SNORD12C and SNORD78 after silencing SNORD12C and SNORD78.

Additional file 1: Table S1.Short hairpin RNAs (shRNAs) sequence against ZFAS1. Table S2.Short hairpin RNAs (shRNAs) sequence against NOP58. Table S3. The reaction assays of qRT-PCR method. Table S4. Primers used in qRT-PCR assays. Table S5. Probes used in situ hybridization (ISH) assay. Table S6. RNA probes used for RNA pull-down assays. Table S7. Primers used in site-specific 2′-O-methylation for RTL-P assay. Table S8. RTL-P assays for rRNA 2′-O-methylation. Table S9. Probes used in site-specific 2′-O-methylation for double-stranded primer based on single-stranded toehold (DPBST) assays. Table S10. Double-stranded primer based on single-stranded toehold (DPBST) assays. Table S11. Data of LncRNAs cluster in Heat map analysis. Table S12. Data of snoRNAs cluster in Heat map analysis. Table S13. Data of mRNAs cluster in Heat map analysis. Table S14. The enriched target genes intersected by ZFAS1, SNORD12C/78 in GO analysis. Table S15. Prognostic information of included colorectal cancer patients (n = 157). Table S16. Multivariate COX regression analysis of the association of ZFAS1 and NOP58 expression with DFS and OS in CRC patients.