Search

Your search keyword '"Gardenia Vargas-Parra"' showing total 11 results
Start Over Author "Gardenia Vargas-Parra" Publisher figshare
11 results on '"Gardenia Vargas-Parra"'

1. MOESM8 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Abstract

Additional file 8: Table S3. Variants identified in the mutational analysis of hereditary cancer genes in case 29.

Published
2019
Full Text
View/download PDF

2. MOESM2 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Subjects
congenital, hereditary, and neonatal diseases and abnormalities, nutritional and metabolic diseases, digestive system diseases
Abstract

Additional file 2: Table S1. Clinical and molecular features of the patients with MLH1 methylated tumors included in this analysis.

Published
2019
Full Text
View/download PDF

3. MOESM12 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Subjects
congenital, hereditary, and neonatal diseases and abnormalities, nutritional and metabolic diseases, neoplasms, digestive system diseases
Abstract

Additional file 12: Table S5. A. Primers and conditions. B. Localization of the probes and regions analyzed in the study of MLH1 methylation. The EMP2AIP1-MLH1 CpG island (colored in dark purple) encompass the MLH1 promoter and intron 1.

Published
2019
Full Text
View/download PDF

4. MOESM3 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Abstract

Additional file 3. Supplementary Methods.

Published
2019
Full Text
View/download PDF

5. MOESM7 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Abstract

Additional file 7: Table S2. MLH1 methylation assessed by MS-MLPA in samples from case 29.

Published
2019
Full Text
View/download PDF

6. MOESM11 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Abstract

Additional file 11: Table S4. Reported patients with MLH1 epigenetic mosaicism at low proportion (≤10%). (*) According to the obtained results the three tumor lesions were clonally related.

Published
2019
Full Text
View/download PDF

7. MOESM10 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Abstract

Additional file 10: Figure S6. Schematic representation of the methodological strategy and summary of the obtained results.

Published
2019
Full Text
View/download PDF

8. MOESM6 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Subjects
digestive system diseases
Abstract

Additional file 6: Figure S4. Immunohistochemical characterization of gastrointestinal tumor lesions from patient 29. All tumor lesions are well-differentiated adenocarcinomas with a variable but not predominant mucinous component. All of them present loss of expression of the cytokeratin markers CK7 and CK20. The transcription factor CDX2 shows strong and diffused positive staining. The expression of the membrane-bound proteins MUC1 and MUC5 is also positive in all tumors but with diffused and lower intensity staining, whereas the MUC2 shows intense, focal and heterogeneous expression. According to this characterization, the three tumors show the same immunohistochemical staining pattern. Objective magnification is 20X for all images. HE, hematoxylin-eosin.

Published
2019
Full Text
View/download PDF

9. MOESM5 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Subjects
congenital, hereditary, and neonatal diseases and abnormalities, nutritional and metabolic diseases, neoplasms, digestive system diseases
Abstract

Additional file 5: Figure S3. MLH1 methylation analysis of the promoter C-region and intron 1 by pyrosequencing. A) and B) Analytical sensitivity analysis for the detection of methylation in MLH1 C-region (A) and intron 1 (B). The detection limits for both regions are 4% and 5% respectively, enabling the detection of positive samples as those with methylation values greater than 4 or 5%. C) and D) Methylation analysis in blood from case 29 and healthy controls (n=10 - 20) for the MLH1 C-region (C) and intron 1 (D). E) and F) Methylation analysis in normal colorectal mucosa from case 29 and Lynch patients (n=4) for MLH1 C-region (E) and intron 1 (F). G) and H) Methylation analysis in normal small bowel mucosa and gastric mucosa from case 29 for MLH1 C-region (G) and intron 1 (H). I) and J) Methylation analysis in normal and gastrointestinal tumor tissues in case 29 for MLH1 C-region (I) and intron 1 (J).

Published
2019
Full Text
View/download PDF

10. MOESM9 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Abstract

Additional file 9: Figure S5. Analysis of structural aberrations in case 29. A) Genome-wide SNP array profiling of blood DNA from case 29 is shown as Circos plots. Circos plot was divided into three concentric circles. Chromosomes are represented at the external circle with their centromeres painted in red. In the middle circle, external allelic peaks mark homozygous SNPs and internal allelic peaks heterozygous ones. Internal circle tracks log2 copy number lane: middle points indicate diploid genomic material; upper points, gains of genomic material and lower points, losses. Patient 29 displayed a diploid pattern throughout her genome without signs of loss-of-heterozygosity. B) CNV analysis in the MLH1 region of patient 29 by custom CGH array. Genes located in the analyzed region are represented at the bottom of the figure. Probes are displayed as green dots in a log2 graph. Gains and losses of genetic material are considered when more than five consecutive probes reach values of 2 or − 2, respectively. No CNV abnormalities were identified.

Published
2019
Full Text
View/download PDF

11. MOESM1 of Highly sensitive MLH1 methylation analysis in blood identifies a cancer patient with low-level mosaic MLH1 epimutation

Author

Dámaso, Estela, Canet-Hermida, Júlia, Gardenia Vargas-Parra, Àngela Velasco, Marín, Fátima, Darder, Esther, Valle, Jesús, Fernández, Anna, Àngel Izquierdo, Mateu, Gemma, Glòria Oliveras, Escribano, Carmen, Piñol, Virgínia, Hugo-Ikuo Uchima, Soto, José, Hitchins, Megan, Farrés, Ramon, Conxi Lázaro, Bernat Queralt, Brunet, Joan, Capellá, Gabriel, and Pineda, Marta

Abstract

Additional file 1. Figure S1. Schematic representation of the origin of the 18 cases included in this study. CRC: colorectal cancer.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results