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Additional file 1: Table S1. Co-relationship between ZDHHC15 and GBM subtype markers in The Cancer Genome Atlas database. Table S2. Co-relationship between ZDHHC15 and GBM subtype markers in The Gene Expression Profiling Interactive Analysis database.

Additional file 5. Amino acid sequence of the Zisp protein. (a) The predicted Zisp domain structure is shown for the transmembrane domain and the Asp-His-His-Cys (DHHC) domain (blue). The DHHC domain of Zisp in zebrafish shows a high degree of conservation with other species. (b) Hydropathy was calculated by Hphob (Kyte–Doolittle scale). Hydrophobic regions that are likely to span membranes are denoted by bars.

Additional file 3. Propofol increases cell death and decreases the number of S-phase cells in retinas at 36 and 72 h postfertilization (hpf). (a) The number of terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive cells (green) in the retinas of propofol-treated embryos (5 μg/ml propofol) at 36 and 72 hpf is increased compared with that in controls. DNA, blue. Scale bar, 25 μm. (b) 5-Ethynyl-2′-deoxyuridine exposure at 36 and 72 hpf decreases the proportion and mislocalization of S-phase cells in the retinas of propofol-treated embryos (5 μg/ml propofol) compared with control retinas. Error bars indicate the standard error of the mean. *, p

Additional file 7. Average and statistical information for Figs. 6 D and I and 7 A, C, E, F, G, H, and I.

Additional file 6. Noggin-1 co-localized with Zisp in the Golgi apparatus. (a, b) Subcellular localization of Zisp (green) in COS cells. The Golgi apparatus (GM130, red), endoplasmic reticulum (Calnexin, red), and mitochondria (Tom20, red). Zisp primarily localized to the Golgi apparatus (a), and a mutant form of Zisp lacking the Asp-His-His-Cys motif within the cytoplasm (b). (c) Zisp co-localized with Noggin-1 into a perinuclear region of COS cells. Scale bar, 10 μm.

Additional file 2: Figure S1. Heatmap showing the molecular subtype marker expression in H4, A172, U87, T98G, U251, and LN18 GBM cell lines. Proneural markers: DCX, DLL3, OLIG2, and ASCL1; mesenchymal markers: CD44, GABRA1, SLC12A5, and TIMP1; Classical markers: FOXO3, AKT2, NES, and EGFR; and Neural markers: SYT1, TGFB1, CHI3L1, and NEFL. Z-scores were calculated from the ΔCt values obtained in the qPCR analysis.

Additional file 4. Expression of the zisp gene during embryogenesis. (a–g) Whole-mount in-situ hybridization of zisp at 10, 16, 24, 32, and 52 h postfertilization (hpf). (a, c, and e–g) Lateral views. (b and d) Dorsal views. (h) Temporal expression profiles of zisp from the eight-cell stage to 3 dpf were determined by reverse transcription polymerase chain reaction (RT-PCR). ef1α was used as an RT-PCR control. Error bars indicate the standard error of the mean.

Additional file 1: Figure S1. qRT-PCR result and correlation analysis with RNA-seq of some selected genes. The cotton Actin gene was used as endogenous gene for normalisation. The error bar represents the SD of three biological replicates.

Additional file 2: Figure S2. Effect of various concentrations of salt (NaCl) on cotton plant (stress time duration was 3 h).

Statistics of Pathway Enrichment of differentially methylated genes (PDF 6Â kb)

Statistics of Pathway Enrichment of differentially methylated genes (PDF 6Â kb)