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Table S6. Galdieria sulphuraria introns that underwent alternative splicing in our studied samples. (PDF 981Â kb)

Figure S1. Two examples of spliceosomal single-gene phylogeny that show different ancestries of red algal spliceosomal genes. (PDF 137Â kb)

Figure S5. Conservation of intron positions in the Galdieria sulphuraria geranylgeranyl transferase beta-subunit gene. (PDF 97Â kb)

Figure S6. Estimation of gains and losses of conserved introns in red algal phylogeny. (PDF 92Â kb)

Table S1. Algal genome and transcriptome data used in this study. (PDF 96Â kb)

Figure S4. GC content and intron density in red algae. (PDF 92Â kb)

Figure S7. The distributions of intron lengths in five red algal species. (PDF 82Â kb)

Table S3. Presence and absence of human spliceosomal machinery-associated proteins in red algae. (PDF 134Â kb)

Figure S8. Intron retention in a Galdieria sulphuraria gene. (PDF 74Â kb)

Supplementary Methods. (PDF 109Â kb)

Table S8. Galdieria sulphuraria genes that were differentially expressed under the heat and cold conditions. (PDF 186Â kb)

Figure S2. The search results for snRNA component of the spliceosome in Galdieria sulphuraria. (PDF 91Â kb)

Table S4. INFERNAL scores and e-values for red algal snRNA genes. (PDF 64Â kb)

Table S5. The intron statistics in red algal and Viridiplantae genomes. (PDF 71Â kb)

Table S7. Galdieria sulphuraria introns that were differentially spliced under the heat and cold conditions. (PDF 226Â kb)

Figure S3. Sequence conservation in Galdieria sulphuraria genes. (PDF 181Â kb)

Figure S9. Phylogenetic trees of UPF1, UPF2, and UPF3. (PDF 97Â kb)