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Additional file 2: Using NC_009915.1 reference sequence as the template of pvmdr1 gene for designment the different primers.

Additional file 6: Fig. S1 The subfigure of SNPs composition at 2014, Fig. S2 The subfigure of SNPs composition at 2020, Fig. S3 The subfigure of SNPs composition at 2021 and Fig. S4 Subfigure of SNPs composition at 2022, respectively.

Additional file 7. The composition of 105 haplotypes identified from 624 pvmdr1 gene sequences.

Additional file 8: Fig.S1 The subfigure of haplotypes composition at 2014, Fig. S2 The subfigure of haplotypes composition at 2020, Fig. S3 The subfigure of haplotypes composition at 2021, Fig. S4 The subfigure of haplotypes composition at 2022, respectively.

Additional file 4: Electrophoresis of PCR amplification products of pvmdr1 gene in P. vivax from vivax malaria cases in Yunnan Province.

Additional file 3: The distribution of vivax malaria cases diagnosed in different years and prefectures.

Additional file 5: Identify true base substitutions.

Additional file 1: Confirmation of malaria case infected with mono-Plasmodium vivax in Yunnan Province.

Additional file 1. The details of nested PCR testing for differentiating between various Plasmodium species.