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Figure S3. Nucleotide alignment of the regions flanking regA in P. sordellii Tet P+ isolates. Sequences for the regA+ isolates SSCC32125, R32462, JGS6961 and SSCC18392 are compared to the regAâ isolate W2945. The direct repeats flanking regA are shown in bold red font and indicated by a black arrow beneath the sequence. The coding region of regA is colored purple. A gap in the alignment representing the majority of regA is indicated by three large dots. The start and stop codons of regA are in bold and annotated on the sequence. The regAâ isolates W3026, AM370 and W2922 have not been included in this alignment; however, a single site identical to the repeat is present for this isolate, equidistant to Tet P, as for W2945. (PDF 238 kb)

Table S1. Matrices displaying the nucleotide identity (%) of the Tet P regulatory region comprising 650â bp directly upstream of the tetA(P) start codon, as well as the amino acid identity (%) of TetA(P) and TetB(P), from isolates of P. sordellii and C. difficile (Cd) compared with that of pCW3 from C. perfringens (Cp) CW92. (PDF 167 kb)

Table S3. Oligonucleotide primers used in PCR. (+) forward primer, (â ) reverse primer. (PDF 89 kb)

Table S2. Bacterial isolates, genomic DNA sequences and sequencing data used in this study. (PDF 274 kb)

Figure S2. Nucleotide alignment of the upstream regulatory regions of Tet P in P. sordellii and C. difficile isolates compared to that of C. perfringens pCW3 from strain CW92. The sequence from P. sordellii isolates that encode Tet P on small plasmids is represented by R28058 (but includes R15892, SSCC37615, R32977 and SSCC19939, Table S1). The sequence from P. sordellii isolates that appear to encode Tet P on the chromosome, with the exception of W2945, is represented by SSCC18392 (but includes SSCC32135, R32462, JGS6961, W3026, AM370 and W2922, Table S1). The beginning of the tetA(P) gene is colored red (red arrow) with the start codon in bold. The predicted ribosome binding sites (RBS) for these sequences are in black bold font. The region corresponding to the C. perfringens pCW3 Tet P promoter, P3, is highlighted in green with the predicted − 10 and − 35 boxes shown in bold. The inverted repeats that correspond to the T1 transcriptional terminator are highlighted in blue and indicated with inverted black arrows. (PDF 274 kb)

Figure S3. Nucleotide alignment of the regions flanking regA in P. sordellii Tet P+ isolates. Sequences for the regA+ isolates SSCC32125, R32462, JGS6961 and SSCC18392 are compared to the regAâ isolate W2945. The direct repeats flanking regA are shown in bold red font and indicated by a black arrow beneath the sequence. The coding region of regA is colored purple. A gap in the alignment representing the majority of regA is indicated by three large dots. The start and stop codons of regA are in bold and annotated on the sequence. The regAâ isolates W3026, AM370 and W2922 have not been included in this alignment; however, a single site identical to the repeat is present for this isolate, equidistant to Tet P, as for W2945. (PDF 238 kb)

Table S2. Bacterial isolates, genomic DNA sequences and sequencing data used in this study. (PDF 274 kb)

Figure S2. Nucleotide alignment of the upstream regulatory regions of Tet P in P. sordellii and C. difficile isolates compared to that of C. perfringens pCW3 from strain CW92. The sequence from P. sordellii isolates that encode Tet P on small plasmids is represented by R28058 (but includes R15892, SSCC37615, R32977 and SSCC19939, Table S1). The sequence from P. sordellii isolates that appear to encode Tet P on the chromosome, with the exception of W2945, is represented by SSCC18392 (but includes SSCC32135, R32462, JGS6961, W3026, AM370 and W2922, Table S1). The beginning of the tetA(P) gene is colored red (red arrow) with the start codon in bold. The predicted ribosome binding sites (RBS) for these sequences are in black bold font. The region corresponding to the C. perfringens pCW3 Tet P promoter, P3, is highlighted in green with the predicted − 10 and − 35 boxes shown in bold. The inverted repeats that correspond to the T1 transcriptional terminator are highlighted in blue and indicated with inverted black arrows. (PDF 274 kb)

Table S3. Oligonucleotide primers used in PCR. (+) forward primer, (â ) reverse primer. (PDF 89 kb)

Table S1. Matrices displaying the nucleotide identity (%) of the Tet P regulatory region comprising 650â bp directly upstream of the tetA(P) start codon, as well as the amino acid identity (%) of TetA(P) and TetB(P), from isolates of P. sordellii and C. difficile (Cd) compared with that of pCW3 from C. perfringens (Cp) CW92. (PDF 167 kb)