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Overlap between experimentally identified and computationally predicted essential genes. Lines 1â 54 details genes that are predicted to be essential in all computationally tested media (100% essentiality score) and are also experimentally identified to be an essential gene under the BAB broth medium. Lines 55â 78 details genes that are predicted to be essential in all computationally tested media (100% essentiality score) but not experimentally identified to be an essential gene under the BAB broth medium. The gene information was collected from http://www.genome.jp on October 18 2016. (DOCX 17Â kb)

Primer sequences used during preparation of libraries for sequencing (DOCX 12Â kb)

Sequences of adapters used during library preparation in this study. (DOCX 11Â kb)

Essential genes identified in different strains of Y. pestis by different methods. Genes in KIM10 identified in in vitro expansion of a himar1-derived transposon library grown on TB agar containing 2.5 mM CaCl2 and 25 μg/ml zeocin at 37 °C, then analysed using the Hidden Markov model (50). Genes in CO92 identified after in vitro growth in BAB broth at both 28 °C and 37 °C, then analysed using the DEM algorithm (14) (DOCX 48 kb)

Overlap between experimentally identified and computationally predicted essential genes. Lines 1â 54 details genes that are predicted to be essential in all computationally tested media (100% essentiality score) and are also experimentally identified to be an essential gene under the BAB broth medium. Lines 55â 78 details genes that are predicted to be essential in all computationally tested media (100% essentiality score) but not experimentally identified to be an essential gene under the BAB broth medium. The gene information was collected from http://www.genome.jp on October 18 2016. (DOCX 17Â kb)