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1. Additional file 1 of Macromolecular crowding links ribosomal protein gene dosage to growth rate in Vibrio cholerae

Author

Soler-Bistué, Alfonso, Aguilar-Pierlé, Sebastián, Garcia-Garcerá, Marc, Marie-Eve Val, Sismeiro, Odile, Varet, Hugo, Sieira, Rodrigo, Krin, Evelyne, Skovgaard, Ole, Comerci, Diego J., Rocha, Eduardo P. C., and Mazel, Didier

Abstract

Additional file 1: Figure S1. The most affected movants, display lower GFP production than the Parental strain at the single cell level. FRAP experiments were performed in LB at 37 °C taking a photo every 2 seconds for at least 5 minutes using the Parental-1120 strain (Parental, violet), the S10Tnp-1120 (red) and S10TnpC2+479 (blue) movants. The parental was also tested in presence of chloramphenicol at MIC (+ Cm). a) A representative plot showing the recovery of fluorescence over time in individual cells. b) The percentage of FRAP at the endpoint of the experiment is shown for all cells tested. Mean with 95% CI is shown. Statistical significance was analyzed by Kruskal-Wallis test (p

Published
2020
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