1. Role of tissue inhibitor of metalloproteinases-1 in the development of autoimmune lymphoproliferation
- Author
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Elisabetta Orilieri, Riccardo Mesturini, Maria Clorinda Mazzarino, Maria Francesca Campagnoli, Elena Boggio, Manuela Indelicato, Ugo Ramenghi, Umberto Dianzani, and Annalisa Chiocchetti
- Subjects
Male ,autoimmune, lymphoproliferative, syndrome ,medicine.medical_specialty ,Adolescent ,Lymphocyte ,Editorials and Perspectives ,Apoptosis ,Biology ,Matrix metalloproteinase ,medicine.disease_cause ,Autoimmunity ,lymphoproliferative ,Internal medicine ,medicine ,Humans ,Osteopontin ,Child ,Tissue Inhibitor of Metalloproteinase-1 ,Hematology ,Autoimmune Lymphoproliferative Syndrome ,Genetic Variation ,autoimmune ,syndrome ,medicine.disease ,medicine.anatomical_structure ,Child, Preschool ,Autoimmune lymphoproliferative syndrome ,Immunology ,biology.protein ,Original Article ,Female ,Antibody - Abstract
Background Inherited defects decreasing function of the Fas death receptor cause autoimmune lymphoproliferative syndrome and its variant Dianzani’s autoimmune lymphoproliferative disease. Analysis of the lymphocyte transcriptome from a patient with this latter condition detected striking over-expression of osteopontin and tissue inhibitor of metalloproteinases-1. Since previous work on osteopontin had detected increased serum levels in these patients, associated with variations of its gene, the aim of this work was to extend the analysis to tissue inhibitor of metalloproteinases-1. Design and Methods Tissue inhibitor of metalloproteinases-1 levels were evaluated in sera and culture supernatants from patients and controls by enzyme-linked immunosorbent assay. Activation- and Fas-induced cell death were induced, in vitro , using anti-CD3 and anti-Fas antibodies, respectively. Results Tissue inhibitor of metalloproteinases-1 levels were higher in sera from 32 patients (11 with autoimmune lymphoproliferative syndrome and 21 with Dianzani’s autoimmune lymphoproliferative disease) than in 50 healthy controls ( P
- Published
- 2010
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