1. [Mutations of the Epstein-Barr virus LMP1 gene mutations in Russian patients with lymphoid pathology and healthy individuals].
- Author
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Pavlish OA, Diduk SV, Smirnova KV, Shcherbak LN, Goncharova EV, Shalginskikh NA, Arkhipov VV, Kichigina MIu, Stepina VN, Belousova NV, Osmanov EA, Iakovleva LS, and Gurtsevich VE
- Subjects
- Amino Acid Sequence, Amino Acid Substitution, Carrier State virology, Genetic Variation, Herpesvirus 4, Human chemistry, Herpesvirus 4, Human pathogenicity, Hodgkin Disease virology, Humans, Infectious Mononucleosis virology, Lymphoma, Non-Hodgkin virology, Molecular Sequence Data, Oncogene Proteins, Viral genetics, Point Mutation, Polymerase Chain Reaction, Russia, Sequence Alignment, Virulence, Epstein-Barr Virus Infections virology, Genes, Viral, Herpesvirus 4, Human genetics, Viral Matrix Proteins genetics
- Abstract
Epstein-Barr virus (EBV) is an etiological agent of a number of benign and malignant human diseases, such as infectious mononucleosis (IM), Hodgkin's lymphoma (HL), and non-Hodgkin's lymphoma (NHL). EBV latent membrane protein 1 (LMP1) gene (recognized as a viral oncoprotein) of various clinical and geographical origin was found to have different types of amino acid mutations affecting its biological activity. Since there was no information on the strain differences in LMP1 of EBV persisting in Russia, the authors made a sequence analysis of LMP1 samples amplified from the biological materials of Russian patients with IM, HL, and NHL and healthy individuals. The studies have shown that LMP1 variants of Russian origin are a mixed heterogeneous group containing both the earlier characterized and presumably new genetic variants. Among the point amino avid substitutions, the mutations S366T, F106Y, 185L, and E328Q associated with the enhanced transforming activity of a LMP1 molecule and its reduced cytotoxicity. There was no specific association between the certain Russian variants of LMP1 and the specific forms of the disease (IM, HL, and NHL).
- Published
- 2008