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Aim: Detection of correlation between quantity of fungi and concentration of polysaccharides in dust of living quarters in Moscow., Materials and Methods: Micromycetes from home dust were isolated by dilution method (1:1000). Polysaccharide concentration in home dust was evaluated by using gel-thromb test (Lal-test, Associates of Cape Cod Inc.)., Results: Correlation between quantity of fungi and concentration of polysaccharide in home dust was not detected., Conclusion: For pyrogenic load evaluation in quarters besides detection of quantity of micromycetes and exposition of microgenic allergens, consideration of concentration of polysaccharide compounds is also actual.

Different detection methods of housing dust allergens (belonging to the tick species Dermatophagoides pteronyssinus, D. farinae) were considered. As shown in this study, the immunochemical method in combination with acarological analysis proved to be most promising. On the basis of the data on the cross activity of tick allergens and changes in their content in dust, grounds were given for the expediency of detection by the immunochemical method with the use of polyclonal antibodies to specially obtained immunogen.

The cross reactivity of the pollen allergens of birch (Betula pendula), hazel (Corylus avelana), alder (Alnus glutinosa), wormwood (Artemisia absinthium), oak (Quercus robur) and ashtree (Fraxinus excelsior) was studied by the methods of the enzyme immunoassay and gel diffusion with the use of rabbit polyclonal antisera (PAS) prepared against birch pollen allergen. PAS was shown to be greatly related to all above-mentioned allergens with the exception of oak pollen allergen. PAS was found capable of forming precipitates in the layer of agar gel only with birch pollen allergen. A proposal was made to use PAS, in view of the established specific features of its immunochemical properties, in the development of test systems for the ecological monitoring of biotopes.

The specific features of the growth of P. aeruginosa in media prepared with the use of Chlorella fermentative hydrolysate as nutrient basis were studied. The accumulation of microbial biomass was shown to depend on increased content of amino nitrogen in the media, while the effectiveness of the pyocyanin formation was linked with decreased content of amino nitrogen and a rise in the amount of sodium chloride.

Literature review is presented on culture media that are regarded as artifical environment for microorganisms permitting modeling of various conditions for revealing cultural, morphological and biochemical properties, characteristic for a given microorganism, as well as manipulations with these conditions.