1. Purification and n-terminal sequencing of two presynaptic neurotoxic PLA2, neuwieditoxin-I and neuwieditoxin-II, from Bothrops neuwiedi pauloensis (jararaca pintada) venom
- Author
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JoséR. Giglio, B. H. Kassab, Sergio Marangoni, L. Rodrigues-Simioni, Marcos H. Toyama, A. M. Soares, Caroline Ribeiro de Borja-Oliveira, and L. Re
- Subjects
lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,Venom ,Toxicology ,Neuromuscular junction ,Dithiothreitol ,chemistry.chemical_compound ,chick biventer cervicis ,lcsh:RA1190-1270 ,neurotoxicity ,lcsh:Zoology ,medicine ,nerve-muscle preparation ,lcsh:QL1-991 ,Patch clamp ,Neuwieditoxin-I ,lcsh:Toxicology. Poisons ,neuromuscular junction ,biology ,Molecular mass ,Bothrops neuwiedi pauloensis ,Anatomy ,biology.organism_classification ,Molecular biology ,In vitro ,Infectious Diseases ,medicine.anatomical_structure ,chemistry ,PLA2 neurotoxin ,presynaptic action ,Bothrops ,Animal Science and Zoology ,Parasitology ,loose patch clamp ,Neuwieditoxin-II ,Acetylcholine ,medicine.drug - Abstract
Two presynaptic phospholipases A2 (PLA2), neuwieditoxin-I (NeuTX-I) and neuwieditoxin-II (NeuTX-II), were isolated from the venom of Bothrops neuwiedi pauloensis (BNP). The venom was fractionated using molecular exclusion HPLC (Protein-Pak 300SW column), followed by reverse phase HPLC (µBondapak C18 column). Tricine-SDS-PAGE in the presence or absence of dithiothreitol showed that NeuTX-I and NeuTX-II had a molecular mass of approximately 14 kDa and 28kDa, respectively. At 10µg/ml, both toxins produced complete neuromuscular blockade in indirectly stimulated chick biventer cervicis isolated preparation without inhibiting the response to acetylcholine, but NeuTX-II reduced the response to KCl by 67.0±8.0% (n=3; p
- Published
- 2007