1. Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
- Author
-
Rommel Rodríguez Burbano, Eleonidas Moura Lima, Cynthia Germoglio Farias de Melo, Plínio Delatorre, João Ricardo Gonçalves de Oliveira, Leonardo Ferreira Soares, Marília de Arruda Cardoso Smith, Otávio Sérgio Lopes, Talitta Dantas de Arruda, Arnaldo Correia de Medeiros, and Carolina Oliveira Gigek
- Subjects
Genetics ,Multidisciplinary ,lcsh:Biotechnology ,Genótipo ,GSTP1 Gene ,Cancer ,Oncogenética ,Single-nucleotide polymorphism ,Biology ,medicine.disease ,Molecular biology ,Gastric Cancer ,GSTP1 ,Carcinoma gástrico ,DSASP method ,genotyping ,lcsh:TP248.13-248.65 ,medicine ,SNP ,Câncer gástrico ,Polimofismo de nucleotídeo ,Allele ,Variants of PCR ,Gene ,CIENCIAS EXATAS E DA TERRA [CNPQ] - Abstract
Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ 2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ 2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.
- Published
- 2015
- Full Text
- View/download PDF