1. Thiol/disulfide status regulates the activity of thiol-containing kinases related to energy homeostasis in rat kidney
- Author
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Itiane Diehl de Franceschi, Genaro Azambuja Athaydes, Virginia Cielo Rech, Nathana J. Mezzomo, Adriana Kessler, Luciane Rosa Feksa, Clovis Milton Duval Wannmacher, and Vandre C. Figueiredo
- Subjects
0301 basic medicine ,Antioxidant ,Cysteamine ,medicine.medical_treatment ,Pyruvate Kinase ,Cystine ,Adenylate kinase ,Kidney ,Random Allocation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Reference Values ,cysteamine ,medicine ,Animals ,Homeostasis ,cystine dimethyl ester ,Disulfides ,Sulfhydryl Compounds ,Rats, Wistar ,lcsh:Science ,Cystine Depleting Agents ,Creatine Kinase ,chemistry.chemical_classification ,Multidisciplinary ,Kinase ,kinases activities ,Adenylate Kinase ,Reproducibility of Results ,030104 developmental biology ,Enzyme ,chemistry ,Biochemistry ,Thiol ,renal energy metabolism ,lcsh:Q ,030217 neurology & neurosurgery ,Pyruvate kinase - Abstract
Considering that thiol-containing enzymes like kinases are critical for several metabolic pathways and energy homeostasis, we investigated the effects of cystine dimethyl ester and/or cysteamine administration on kinases crucial for energy metabolism in the kidney of Wistar rats. Animals were injected twice a day with 1.6 µmol/g body weight cystine dimethyl ester and/or 0.26 µmol/g body weight cysteamine from the 16th to the 20th postpartum day and euthanized after 12 hours. Pyruvate kinase, adenylate kinase, creatine kinase activities and thiol/disulfide ratio were determined. Cystine dimethyl ester administration reduced thiol/disulfide ratio and inhibited the kinases activities. Cysteamine administration increased the thiol/disulfide ratio and co-administration with cystine dimethyl ester prevented the inhibition of the enzymes. Regression between the thiol/disulfide ratio, and the kinases activities were significant. These results suggest that redox status may regulate energy metabolism in the rat kidney. If thiol-containing enzymes inhibition and oxidative stress occur in patients with cystinosis, it is possible that lysosomal cystine depletion may not be the only beneficial effect of cysteamine administration, but also its antioxidant and thiol-protector effect.
- Published
- 2017