1. HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC)
- Author
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Michael R. Borenstein, Kim Cherng-Ju, and Cristina Duarte Vianna-Soares
- Subjects
Aqueous solution ,Materials science ,Chromatography ,Mechanical Engineering ,macromolecules separation ,Size-exclusion chromatography ,size exclusion chromatography ,Condensed Matter Physics ,Methacrylate ,hydrophilic acrylate packing materials ,chemistry.chemical_compound ,Dextran ,chemistry ,Mechanics of Materials ,lcsh:TA401-492 ,Copolymer ,lcsh:Materials of engineering and construction. Mechanics of materials ,General Materials Science ,Particle size ,Methyl methacrylate ,Porosity - Abstract
HEMA/MMA/EDMA (2-hydroxyethyl methacrylate/methyl methacrylate/ethyleneglycol methacrylate) copolymer beads have been synthesized for use in aqueous SEC. This porous chromatographic support consisted of a large average particle size (250 µm) and contained macropores with a median pore size of approximately 880 Å. The material was packed in glass and stainless columns to assess their chromatographic performance. The packed columns were calibrated using narrow molecular weight (MW) distribution standards (dextran) and exhibited a range of molecular weight separation between 40,000 and 2,000,000 daltons. The packing material showed the ability to separate large molecules through the size exclusion mechanism.
- Published
- 2005
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