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Enteric fermentation process is of concern worldwide for its contribution to global warming. Methanogenesis process represents besides its contribution to greenhouse gases emissions an energy loss to the animal. In the last years, new strategy has been evaluated whether plant secondary metabolites can be used as natural additives to reduce ruminal methane emissions. The present study investigated the effects of trans-cinnamic, caffeic, p-coumaric acids and catechin hydrate, four plant secondary metabolites (PSMs) on methane production and fermentation in in vitro ruminal cultures. The four PSMs were added anaerobically in a 6 mM concentration to 100 ml serum bottles containing 500 mg grass hay as substrate, 10 ml rumen fluid collected from a fistulated sheep and 40 ml 141 DSM culture medium. The bottles were incubated at 39 °C. The results showed that caffeic (p = 0.058) and p-coumaric (p = 0.052) acids tended to decrease methane production in comparison to control but the decrease was not statistic significantly at α= 0.05. The other two PSMs had no significant effect on methane production. Addition of PSMs did not affected the total gas volume, the pH and VFAs profile (P>0.05) in relation to the control (no PSM added). Caffeic and p-coumaric acids in 6 mM concentration showed promising effects for decreasing ruminal methane emissions without affecting ruminal fermentation parameters, further experiments with other concentrations needs to be done. [ABSTRACT FROM AUTHOR]

The investigations on the incidence and intensity of parasitism in some endoparasytes in sheep performed on 376 animals (260 sheep and 116 young adult sheep) Turcana breed, Gilau area, Cluj County, indicated an increased incidence in trihostrongilidys of 72.7% in young sheep and 65.0% in adult sheep. The incidence of monesya had different values depending on age group, 45.5% at young sheep and 10.0% in adult sheep. The data obtained regarding the prevalence of hepatobiliary trematodsys of sheep in the study, indicates similar values for the two categories, 27.2% young sheep and 30.0% for adult sheep. Instead the extensivity of lung strongilatosys showed notable differences between the two groups of animals, 18.1% at young sheep and 35.0% in adult sheep. The cop microscopic pollution degree with trihostrongily eggs in correlation with age group, indicated a dominance of low infestation (50% young sheep and 61.5% adult sheep). In exchange, the data obtained on parasitism intensity with cestode oncospheres expresses a dominance of medium infestation (60.0%) in young sheep and the low (10.0%) in adult sheep. The intensity level of Hepatobiliary trematodosys parasitism in the young sheep showed a dominance of small and medium infestations (37.5%) and in adult sheep is dominant only at low infestations (45.4%). The larvae strongilatosys parasitism intensity in the lung detected similar values in the two age groups (50.0% in young sheep and 17.2% in adult sheep). [ABSTRACT FROM AUTHOR]

Research conducted in dynamic, in 2005-2010 period, regarding the incidence of outbreaks of equine infectious anaemia in Mures County, on a cumulative total of 925 actual horses from households in the commune and the 2 villages belonging and serologically tested (Coggins test) showed a significant decline in positive serologically diagnosed cases, 20 positive cases in 2005 (10.9%) to 3 positive cases in 2010 (0.25%). Cumulative distribution of outbreaks of equine infectious anaemia in the commune and the 2 villages belonging showed variation, in the commune 16 cases (5.04%), in the I village 26 cases (7.10%) and in the II village 10 cases (3.87%). Assessing responsiveness to equine infectious anaemia correlated with sex, in the time reported showed a higher receptivity in males (5.7%) than females (5.5%). Also was found a higher responsiveness to infectious disease between age group 6-10 years (3.4%) compared with the age group between 11-15 years (2.1%). [ABSTRACT FROM AUTHOR]

Clinical and laboratory investigations on the incidence and intensity of sarcoptic mange in a herd of 156 young cattle and histo-pathological picture of changes in the skin, highlights the following: anatomical-clinical examination performed revealed that 47.4% of young cattle have examined clinical anatomical changes visible (crusts, depilation, itching and scratching), with significant differences related to age and type of anatomy-clinical manifestation. The incidence of sarcoptic mange by microscopic examination, is ranging from 34.75 (category 0 - 6 months) 38.0% (category 6-12 months) with an average of 36.5%. The intensity of parasitism with Sarcoptes scabiei, var. bovis is correlated with the age. Thus the 0-6 months category predominate weak infestation (44.0%), while the 6-12 months category predominate large and medium infestation (40.6%). Histo-pathologically, the sections made in the skin and colored shows the presence of parasites in exfoliate epithelium with a low limfo granulocytes infiltration. [ABSTRACT FROM AUTHOR]

The biological material of buffaloes in this area shows favorable characteristics in the direction of milk production with a total length of lactation almost close to the normal one, but the interval between births is quite high. Pure milk fat content on total lactation is 6.98% and 6.96% for normal lactation, with variations from the first lactation of 7.27% to 6.86% in the ninth lactation. The time of the dry period shows variation between 80.84 and 142.84 days and calving interval between 375.91 and 435.48 days. [ABSTRACT FROM AUTHOR]

Consumer preferences regarding the various species of fish or aquatic organisms are highly variable. The criteria by which they orient are represented by: the price, organoleptic characteristics, healing and nutritional properties of meat. Today it is known that a high consumption of fish meat has a beneficial role in human health. Moreover, statistics indicates a high level of life expectancy in countries with tradition in terms of fish consumption, e.g. North- European and Asian countries. Statistics shows a high consumption of ocean fish and different species of salmonid family. The culture and intensive fish farming represents an alternative to the requirements of the fish market. The salmonids farmers focus their efforts to obtain high yields of high quality, in conditions of maximum economic efficiency. In Romania, the predominant specie encountered in salmonis farms is rainbow trout (Oncorhynchus mykiss). It is successfully reared because of its plasticity and resistance to changes in environmental conditions and disease, and efficient feed-conversion. For restocking mountain water with biological material, some trout farms operate successfully brown trout (Salmo trutta fario), a less effective specie for meat production, due to slow growth and development and low resistance to changing environmental factors. Profitability of fish production depends on the propagation processes, fish growth and developments, and supplying optimal environmental conditions for enhancement of the biological potential. The artificial reproduction of salmonids, involves several technological operations for achieving outstanding results on fisheries production. Of these operations, critical is the selection and improvement of breeding. [ABSTRACT FROM AUTHOR]

Investigations were done on 7 alive samples in agonic or death stage, taken from an extensive exploitation pond, in north-east Transylvanian zone, with an water surface of 0,2 ha and a total fish quantity of about 200 kg, second summer carp of an average weight of 300 g/individual. As a result of clinical, bacterioscopy and bacteriology and also necropsy laboratory exam it were put in evidence some aspects. So, clinical exam has revealed, by the presence of integument hemorrhagic ulcerous lesions, also the presence of some crustacaee ectoparasites, Argulus foliaceus species, which have made easier the disease spreading in whole effective. Necropsy exam puts in evidence, anatomyclinic, the presence of integument hemorrhagic lesions under a point or diffuse form, but also ulcers and muscle - cutaneous necrosis. As a following of bacterioscopy exam of smears done from pathologic material taken in cutaneous lesions and colored by Gram method it was ascertained the presence of a bacterial polymorph flora, of bacillus and cocobacillus type, Gram negative. Bacteriologic exam (cultural), realized on ordinary culture mediums (sauce, water) and selective mediums (agar with blood) isolates in pure culture germs from Aeromonas kind, basis on morphological and cultural characters, which are framed in Aeromonas genus patterns described by speciality literature. As a following of biochemical characters exam, with the help of multitest API20E system it was established that Aeromonas spp. strains isolated, belong to hydrophila group 1 species, species which is responsible of bacterial hemorrhagic septicemia performing in carp. By testing the sensitiveness to antibodies and chemiotherapy in strains which have been found as diagnosis (diffusiometric method of antibiogram), it was ascertained that group 1 Aeromonas hydrophila strains, isolated from disease pesthole, were in decreasing order sensitive to : Eurofloxocin, Florfenicol (Floron) and Oxitetracycline and resistant to Ampiciline, Amoxicilin, Eritromicin. [ABSTRACT FROM AUTHOR]

Clinical and paraclinical investigations (morpho-pathologic, bacterioscopic, bacteriologic and sensitiveness test to antibodies) done on 20 fish (rainbow trout), dead or in agony state, taken from a furunculosis pesthole in Salmonidae and proceeded from an intensive exploitation unit of trout, in Cluj county, put in evidence the following aspects :To clinical exam, in breeding basins, it appears individuals (5 %) which have presented locomotor troubles, listless and untidy swimming in water surface, lateral swimming anorexia, and on tegument it is observed diffuse hemorrhagic zones, lost of fish scale and necrosis of fins. It appears also secondary infections with fungi of Saprolegnia genus under the form of a white- dirty, downy film. From the 20 trut corpses were isolated 2 bacterial strains, from anterior kidney and from raclage in fin basis, with congestive lesions. Bacteria isolated are developing only after an incubation at 20 - 25° C and does not grow up to 37° C, and to microscopic exam directly from sauce, the mobility is absent Classic biochemical tests put in evidence the following positive biochemic properties: cataloze, oxidaze, indolal and the presence of hemolytic activity. Biochemical properties testing on API20E gallery also puts in evidence properties as : positive gelatinousis and sucraze fermentation. Based on bacterial strains development isolated only to 20 - 25 °C and the above mentioned biochemic properties, respectively the absence of pigment, bacterial strains isolated are appointed in Aeromonas genus, Aeromonas salmonicida species, achromogenes subspecies. By sensitiveness test in different medicine substances, using antibiogram technique, bacterial strains isolated from pesthole are sensitive in a decreasing order to: nalidixic acid, oxitetracycline, florfenicol and eurofloxacin and resistant to ampicyline, amoxiclav and colistin. [ABSTRACT FROM AUTHOR]

Investigations carried out on a herd of 485 sheep (ovine adult and youth), a private unit of household type, regarding the incidence of pod dermatitis and the picture in dynamic of clinical anatomical changes in the period January-April 2011, highlights the increasing frequency of their 2.2% in January to 2.7% in February, 3.4% in March and 4.6 in April. Of all disease, are dominant lesions of pod dermatitis with necrobacilium (66.1%), followed by lesions of felon (15.9%), suppurative pod dermatitis (12.9%) and other causes (6.4%). [ABSTRACT FROM AUTHOR]

There were collected samples of mammary gland tissue from Turcana breed sheep, nearly aged, in lactation stage and with similar mammary gland volume/conformation. By histological and stereological evaluation were determined the relative areas of glandular parenchyma (mammary alveoli, excretion canaliculli system) and stroma (connective tissue, blood vessels, nerves). Before histological sample collecting was determined the average milk production resulted from three successive milking days. The results revealed positive correlations between average milk production and glandular parenchyma area and negative correlations between average milk production and connective stroma area from mammary gland. [ABSTRACT FROM AUTHOR]