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OBJECTIVE ： To establish the method for content determination of complanatuside and paeoniflorin in juemingtuizhang oral liquid by HPLC. METHODS： (1) To determine the content of complanatuside. The column was Aglient ZORBAX Eclipse Plus C18(2. 1 mm x50 mm,l. 8 μm)，the mobile phase was acetonitrile-0. 1% phosphoric acid solution ( l7 : l7 = 21 : 79 ) , and the detective wavelength was 266 nm. (2 ) To determine the content of paeoniflorin. The column was Diamonsil C18(2) (250 mm x4. 6 mm,5 μm) , the mobile phase was acetonitrile-0. 1% phosphoric acid solution (V- 1/ = 14*86) , and the detective wavelength was 230 nm. RESULTS ： The standard curve regression equation of complanatuside was F = 21 338 Z - 29. 63 ,7* = 0• 999 6(71 = 6) , the sample size was well linear in the range of 1-32 [xg, and the transfer rate was 80. 31% . In addition, the standard curve regression equation of complanatuside was F = 12 467 X - 41. 99，r =0• 999 9 (Ti = 6 )，the sample size was well linear in the range of 1-48 μg, and the transfer rate was 64. 69%. CONCLUSIONS： This method can be used for accurate content determination of complanatuside and paeoniflorin in juemingtuizhang oral liquid, and provide basis for quality control of juemingtuizhang oral liquid. [ABSTRACT FROM AUTHOR]