1. Lung exposure to nanoparticles modulates an asthmatic response in a mouse model
- Author
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V. De Vooght, Katrien Luyts, Salik Hussain, Jeroen Vanoirbeek, Ben Nemery, Erik Verbeken, David Dinsdale, Sonja Boland, Johan A. Martens, Francelyne Marano, Leen C.J. Thomassen, and Peter Hoet
- Subjects
Pulmonary and Respiratory Medicine ,Lung ,biology ,Toluene diisocyanate ,medicine.diagnostic_test ,business.industry ,medicine.medical_treatment ,Respiratory disease ,Inflammation ,Histology ,respiratory system ,Pharmacology ,Immunoglobulin E ,medicine.disease ,respiratory tract diseases ,chemistry.chemical_compound ,medicine.anatomical_structure ,Cytokine ,Bronchoalveolar lavage ,chemistry ,Immunology ,biology.protein ,Medicine ,medicine.symptom ,business - Abstract
The aim of this study was to investigate the modulation of an asthmatic response by titanium dioxide (TiO₂) or gold (Au) nanoparticles (NPs) in a murine model of diisocyanate-induced asthma. On days 1 and 8, BALB/c mice received 0.3% toluene diisocyanate (TDI) or the vehicle acetone-olive oil (AOO) on the dorsum of both ears (20 μL). On day 14, the mice were oropharyngeally dosed with 40 μL of a NP suspension (0.4 mg·mL⁻¹ (∼0.8 mg·kg⁻¹) TiO₂ or Au). 1 day later (day 15), the mice received an oropharyngeal challenge with 0.01% TDI (20 μL). On day 16, airway hyperreactivity (AHR), bronchoalveolar lavage (BAL) cell and cytokine analysis, lung histology, and total serum immunoglobulin E were assessed. NP exposure in sensitised mice led to a two- (TiO₂) or three-fold (Au) increase in AHR, and a three- (TiO₂) or five-fold (Au) increase in BAL total cell counts, mainly comprising neutrophils and macrophages. The NPs taken up by BAL macrophages were identified by energy dispersive X-ray spectroscopy. Histological analysis revealed increased oedema, epithelial damage and inflammation. In conclusion, these results show that a low, intrapulmonary doses of TiO₂ or Au NPs can aggravate pulmonary inflammation and AHR in a mouse model of diisocyanate-induced asthma.
- Published
- 2010