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Your search keyword '"immunomagnetic assay"' showing total 2 results
Start Over Descriptor "immunomagnetic assay" Publisher escholarship, university of california
2 results on '"immunomagnetic assay"'

1. Strong and oriented conjugation of nanobodies onto magnetosomes for the development of a rapid immunomagnetic assay for the environmental detection of tetrabromobisphenol-A

Author

He, Jinxin, Tian, Jiesheng, Xu, Junjie, Wang, Kai, Li, Ji, Gee, Shirley J, Hammock, Bruce D, Li, Qing X, and Xu, Ting

Subjects
Analytical Chemistry, Biological Sciences, Chemical Sciences, Biotechnology, Prevention, Bacteria, Biological Assay, Environmental Monitoring, Enzyme-Linked Immunosorbent Assay, Inhibitory Concentration 50, Limit of Detection, Magnetics, Microscopy, Electron, Transmission, Polybrominated Biphenyls, Time Factors, Oriented conjugation, Nanobody, Bacterial magnetic particles, Immunomagnetic assay, Tetrabromobisphenol-A, Environmental detection, Engineering, Biological sciences, Chemical sciences
Abstract

Variable domain of heavy chain antibody (nanobody, Nb) derived from camelids is an efficient reagent in monitoring environmental contaminants. Oriented conjugates of Nbs and bacterial magnetic particles (BMPs) provide new tools for the high-throughput immunoassay techniques. An anti-tetrabromobisphenol-A (TBBPA) Nb genetically integrated with an extra cysteine residue at the C terminus was immobilized onto BMPs enclosed within the protein membrane, using a heterobifunctional reagent N-succinimidyl-3-(2-pyridyldithiol) propionate, to form a solid BMP-Nb complex. A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) based on the combination of BMP-Nb and T5-horseradish peroxidase was developed for the analysis of TBBPA, with a total assay time of 30 min and a half-maximum signal inhibition concentration (IC50) of 1.04 ng/mL in PBS (pH 10, 10% methanol and 0.137 moL/L NaCl). This assay can even be performed in 100% methanol, with an IC50 value of 44.3 ng/mL. This assay showed quantitative recoveries of TBBPA from spiked canal water (114-124%) and sediment (109-113%) samples at 1.0-10 ng/mL (or ng/g (dw)). TBBPA residues determined by this assay in real canal water samples were below the limit of detection (LOD) and in real sediments were between

Published
2018

2. Strong and oriented conjugation of nanobodies onto magnetosomes for the development of a rapid immunomagnetic assay for the environmental detection of tetrabromobisphenol-A

Author

Bruce D. Hammock, Kai Wang, Jiesheng Tian, Shirley J. Gee, Jinxin He, Junjie Xu, Ji Li, Qing X. Li, and Ting Xu

Subjects
Time Factors, Polybrominated Biphenyls, Enzyme-Linked Immunosorbent Assay, 02 engineering and technology, 01 natural sciences, Biochemistry, Electron, Article, Analytical Chemistry, chemistry.chemical_compound, Inhibitory Concentration 50, Magnetics, Engineering, Microscopy, Electron, Transmission, Oriented conjugation, Limit of Detection, medicine, Transmission, Detection limit, Residue (complex analysis), Microscopy, Chromatography, Heavy-chain antibody, biology, medicine.diagnostic_test, Bacteria, 010401 analytical chemistry, Environmental detection, Immunomagnetic assay, Biological Sciences, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Bacterial magnetic particles, chemistry, Reagent, Immunoassay, Tetrabromobisphenol-A, Chemical Sciences, biology.protein, Nanobody, Tetrabromobisphenol A, Biological Assay, 0210 nano-technology, Peroxidase, Conjugate, Environmental Monitoring
Abstract

Variable domain of heavy chain antibody (nanobody, Nb) derived from camelids is an efficient reagent in monitoring environmental contaminants. Oriented conjugates of Nbs and bacterial magnetic particles (BMPs) provide new tools for the high-throughput immunoassay techniques. An anti-tetrabromobisphenol-A (TBBPA) Nb genetically integrated with an extra cysteine residue at the C terminus was immobilized onto BMPs enclosed within the protein membrane, using a heterobifunctional reagent N-succinimidyl-3-(2-pyridyldithiol) propionate, to form a solid BMP-Nb complex. A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) based on the combination of BMP-Nb and T5-horseradish peroxidase was developed for the analysis of TBBPA, with a total assay time of 30 min and a half-maximum signal inhibition concentration (IC(50)) of 1.04 ng/mL in PBS (pH 10, 10% methanol and 0.137 moL/L NaCl). This assay can even be performed in 100% methanol, with an IC(50) value of 44.3 ng/mL. This assay showed quantitative recoveries of TBBPA from spiked canal water (114–124%) and sediment (109–113%) samples at 1.0–10 ng/mL (or ng/g (dw)). TBBPA residues determined by this assay in real canal water samples were below the limit of detection (LOD) and in real sediments were between < LOD and 23.4 ng/g (dw). The BMP-Nb based ELISA shows promising application in environmental monitoring.

Published
2018

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