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1. Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II

Author

Sierra, RG, Gati, C, Laksmono, H, Dao, EH, Gul, S, Fuller, F, Kern, J, Chatterjee, R, Ibrahim, M, Brewster, AS, Young, ID, Michels-Clark, T, Aquila, A, Liang, M, Hunter, MS, Koglin, JE, Boutet, S, Junco, EA, Hayes, B, Bogan, MJ, Hampton, CY, Puglisi, EV, Sauter, NK, Stan, CA, Zouni, A, Yano, J, Yachandra, VK, Soltis, SM, Puglisi, JD, and DeMirci, H

Abstract

We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (

Published
2015

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