1. Quantitative Human Immunodeficiency Virus (HIV)-1 Antibodies Correlate With Plasma HIV-1 RNA and Cell-associated DNA Levels in Children on Antiretroviral Therapy
- Author
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McManus, Margaret, Henderson, Jennifer, Gautam, Anita, Brody, Robin, Weiss, Eric R, Persaud, Deborah, Mick, Eric, Luzuriaga, Katherine, Gay, Hannah, Borkowsky, William, van Dyke, Russell, Stechenberg, Barbara, McIntosh, Kenneth, Farley, John, Pelton, Stephen, Petru, Ann, Spector, Stephen, Bonagura, Vincent, Cunningham, Coleen, Deveikis, Audra, Ruff, Andrea, Shearer, William, McKinney, Ross, Bakshi, Saroj, Rubio, Thomas, Rich, Kenneth, Gaur, Sunanda, Johnson, George, Rana, Sohail, Sleasman, John, and Febo, Irma
- Subjects
Medical Microbiology ,Biomedical and Clinical Sciences ,Immunology ,Sexually Transmitted Infections ,Clinical Trials and Supportive Activities ,Pediatric AIDS ,Pediatric ,HIV/AIDS ,Clinical Research ,Infectious Diseases ,6.1 Pharmaceuticals ,2.2 Factors relating to the physical environment ,Infection ,Good Health and Well Being ,Anti-HIV Agents ,Antigens ,Viral ,Cohort Studies ,DNA ,Viral ,HIV Antibodies ,HIV Infections ,HIV-1 ,Humans ,Immunoglobulin G ,Infant ,Infant ,Newborn ,Puerto Rico ,RNA ,Viral ,Sustained Virologic Response ,United States ,pediatric early antiretroviral therapy ,HIV-1 persistence ,HIV-1 quantitative antibodies ,PACTG 356 Investigators ,Biological Sciences ,Medical and Health Sciences ,Microbiology ,Clinical sciences - Abstract
BackgroundThis study measured serial plasma human immunodeficiency virus (HIV)-1-specific antibody (Ab) levels in children who initiated antiretroviral therapy (ART) prior to 2 years of age, and evaluated their relationship to peripheral blood HIV-1 RNA and DNA levels.MethodsWe studied 46 HIV-1-infected children, stratified by age at ART initiation (3 mo-2 years, late therapy [LT]) and by virologic response (R) or non-response (NR), before and up to 4 years following ART. We studied 20 HIV-1-uninfected children born to HIV-1-infected mothers (seroreverters [SR]) as controls. Plasma immunoglobulin G (IgG) Ab levels directed against HIV-1 envelope (gp160, gp41), gag (capsid, p24; matrix, p17), reverse transcriptase (p66/51), and integrase (p31) were serially measured using quantitative enzyme-linked immunosorbent assays. HIV-1 Ab rates of decline were estimated over the first 15 months of the study.ResultsThe HIV-1 Ab rates of decline in the ET-R group were similar to those in the SR group for all Ab specificities, except for p17 (P = .01). Ab decline rates in the LT-R group and the NR group were significantly slower than in the SR group for all tested Ab specificities. After 1 year of age, Ab levels to p31 and p17 were significantly associated with HIV-1 RNA levels (P < .001); Ab levels to gp160 (P < .001) and gp41 (P < .001) were significantly associated with cell-associated HIV-1 DNA levels.ConclusionsQuantitative HIV-1-specific Ab levels may be useful for screening children on ART for viral suppression or for residual, cell-associated HIV-1 DNA levels.Clinical trials registrationNCT00000872.
- Published
- 2019