1. Ribosome and transcript copy numbers, polysome occupancy and enzyme dynamics in Arabidopsis
- Author
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Waltraud X. Schulze, Johann M. Rohwer, Mark Stitt, Björn Usadel, Maria Piques, Yves Gibon, Melanie Höhne, Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, Station de physiologie végétale, Institut National de la Recherche Agronomique (INRA), and Stellenbosch University
- Subjects
0106 biological sciences ,développement végétal ,[SDV]Life Sciences [q-bio] ,Gene Dosage ,génomique fonctionnelle ,translation ,01 natural sciences ,Ribosome ,Gene Expression Regulation, Plant ,Arabidopsis ,Protein biosynthesis ,skin and connective tissue diseases ,2. Zero hunger ,chemistry.chemical_classification ,0303 health sciences ,Applied Mathematics ,Translation (biology) ,Darkness ,Circadian Rhythm ,ribosome ,Computational Theory and Mathematics ,Biochemistry ,General Agricultural and Biological Sciences ,Subcellular Fractions ,Information Systems ,traduction ,RT-PCR QUANTITATIVE ,Biology ,Article ,General Biochemistry, Genetics and Molecular Biology ,physiologie végétale ,03 medical and health sciences ,Polysome ,Respiration ,RNA, Messenger ,BIOLOGIE VEGETALE ,Codon ,030304 developmental biology ,polysomes ,General Immunology and Microbiology ,quantitative RT–PCR ,Arabidopsis Proteins ,biology.organism_classification ,Plant Leaves ,arabidopsis ,Enzyme ,chemistry ,Polyribosomes ,Protein Biosynthesis ,sense organs ,polysome ,010606 plant biology & botany - Abstract
Plants are exposed to continual changes in the environment. The daily alternation between light and darkness results in massive recurring changes in the carbon budget, and leads to widespread changes in transcript levels. These diurnal changes are superimposed on slower changes in the environment. Quantitative molecular information about the numbers of ribosomes, of transcripts for 35 enzymes in central metabolism and their loading into polysomes is used to estimate translation rates in Arabidopsis rosettes, and explore the consequences for important sub-processes in plant growth. Translation rates for individual enzyme are compared with their abundance in the rosette to predict which enzymes are subject to rapid turnover every day, and which are synthesized at rates that would allow only slow adjustments to sustained changes of the environment, or resemble those needed to support the observed rate of growth. Global translation rates are used to estimate the energy costs of protein synthesis and relate them to the plant carbon budget, in particular the rates of starch degradation and respiration at night. Molecular Systems Biology 5: 314; published online 13 October 20009; doi:10.1038/msb.2009.68
- Published
- 2009
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