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Ethnopharmacological Relevance: Kheaw-Hom (KH) remedy, a Thai traditional medicine (TTM) on the National List of Essential Medicines, has long been clinically used to treat fever and inflammation in children. However, no in vitro or in vivo anti-inflammatory or bioactive compound studies are published in the literature., Aims of the Study: To explore the in vitro and in vivo anti-inflammatory activities of KH remedy and its bioactive compound and analyze relationships between flavor and ethnopharmacological activities of plant components in KH remedy according to TTM theory., Materials and Methods: Ethyl p-methoxycinnamate (EPMC), a bioactive compound of KH remedy was analyzed using high performance liquid chromatography (HPLC). In vitro anti-inflammatory activities of ethanolic extract (KHE), aqueous extract (KHA), acid-hydrolysis of KHA (KHA-h), acid-hydrolysis of KH powder (KHP-h), and EPMC were investigated using lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin E 2 (PGE 2 ), and tumor necrosis factor-alpha (TNF-α) production in murine macrophage RAW 264.7 cells. In vivo anti-inflammatory activities of KH powder (KHP) and KHE were determined using carrageenan-induced paw edema and ethyl phenylpropiolate (EPP)-induced ear edema in rats and PGE 2 production in tissue samples was examined., Results: KHP-h showed the highest EPMC content (21.33 ± 1.08 mg/g of extract) and inhibited PGE 2 , NO, and TNF-α production with IC 50 values of 11.92 ± 0.21, 30.61 ± 3.12, and 56.71 ± 2.91 μg/mL, respectively, followed by KHE and KHA-h while KHA did not. EPMC, a bioactive compound of KH remedy showed high anti-inflammatory activities through three pathways. KHP oral administration (100 mg/kg) significantly minimized rat paw inflammation at 1, 2, and 3 h while KHE (100 mg/kg) noticeably reduced at 2 and 3 h. KHP (100, 200, and 400 mg/kg) and KHE (100 mg/kg) significantly inhibited PGE 2 production. KHP (1% w/v) notably reduced rat ear edema at 30, 60, and 120 min whereas KHE at all concentrations decreased swelling at 120 min. KHP and KHE at all doses significantly inhibited PGE 2 production. Cool flavor was the main KH remedy flavor. Spicy plant components and some fragrant components showed high anti-inflammatory activity., Conclusions: Results from the in vivo study strongly paralleled the in vitro study. These findings support the rational use of KH remedy according to TTM theory for fever treatment and inflammation in children., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Ethnopharmacological Relevance: Sa-Tri-Lhung-Klod (ST) remedy is a Thai traditional remedy used in obstetrics and gynecology to reduce inflammation and nourish the body post-partum. In Thai traditional medicine (TTM), there is a theory of formulating drugs based on the four elements and the tastes of herbs for use in treating diseases., Aims of Study: To determine relationships between taste theory and pharmacological properties for the anti-inflammatory effect of ST remedy and its chemical constituent components. To evaluate anti-inflammatory activity and also investigate the HPLC fingerprint of ST extracts., Materials and Methods: ST remedy was extracted by maceration in 95% ethanol (STE) and decoction in distilled water (STW). ST extracts were evaluated for anti-inflammatory activity by nitric oxide inhibitory assay in RAW264.7 cells, carrageenan-induced rat paw edema, and prostaglandin E 2 inhibitory assay in inflamed rat paw tissue. In addition, the chemical constituent fingerprints of ST extracts were examined using HPLC., Results: STE contained seven main chemical compounds, and STW demonstrated only one identifiable chemical compound. The STE and STW displayed potent NO inhibitory activity with an IC 50 value of 20.59 ± 0.03 and 52.93 ± 0.90 μg/mL, respectively. Moreover, the STE and STW (at doses of 100 - 400 mg/kg) promoted significant inhibition of inflammation in carrageenan-induced paw edema in rats (20.81 - 38.25%). Additionally, the STE (200 - 400 mg/kg) and STW (100 - 400 mg/kg) significantly reduced PGE 2 levels in inflamed rat paw tissue., Conclusion: These findings suggest that the spicy, astringent, sweet, and fragrant taste of the ST remedy used to treat post-partum inflammation encompass constituents with potent anti-inflammatory activity. STE and STW possess anti-inflammatory properties and effectively inhibit the production of NO and PGE 2 . Results confirm the use of the ST remedy for treating inflammatory diseases in the post-partum period according to TTM knowledge., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Crown Copyright © 2023. Published by Elsevier B.V. All rights reserved.)

Ethnopharmacological Relevance: Prasachandaeng (PSD) remedy is a famous antipyretic drug for adults and children in Thai traditional medicine used and is described in Thailand's National List of Essential Medicine. Relationship between the taste of this herbal medicine, ethnopharmacological used and its pharmacological properties was reviewed., Aims of Study: Since there has been no scientific report on the antipyretic activity of PSD, aim of this study was to investigate the efficacy related antipyretic drug of the remedy and its 12 herbal ingredients. It involved quality evaluation of raw materials, extraction of PSD and its ingredients, in vitro evaluation of their inhibitory activities on fever mediators, i.e. NO and PGE 2 production in murine macrophage (RAW 264.7) cell line stimulated by lipopolysaccharide, and its stability study of the 95% ethanolic extract of PSD remedy., Materials and Methods: PSD remedy was extracted by maceration with 50% and 95% ethanol (PSD50 and PSD95), by decoction with distilled water (PSDW), and hydrolysis of PSDW with 0.1 N HCl (PSDH). The 12 plant ingredients were extracted with 95% ethanol. Quality evaluation of PSD ingredients was performed according to the standard procedures for the quality control of herbal materials. The inhibitory activity on nitric oxide production was determined by the Griess reaction and the inhibition of prostaglandin E 2 production was determined using the ELISA test kit., Results: PSD ingredients passed the quality standard stipulated for herbal materials. PSD95 exhibited the highest inhibitory activities on the production of NO and PGE 2 with the IC 50 values of 42.40 ± 0.72 and 4.65 ± 0.76 μg/mL, respectively. A standard drug acetaminophen (ACP) exhibited inhibition of NO and PGE 2 production with the IC 50 values of 99.50 ± 0.43 and 6.110 ± 0.661 μg/mL, respectively. The stability study was suggested two years shelf-life of PSD95. This is the first report on the activity related antipyretic activity of PSD remedy and its ingredients against two fever mediators, NO and PGE 2 ., Conclusion: The results suggested that the 95% ethanolic extracts of PSD remedy and some of its ingredients, were better than ACP in reducing fever. PSD should be further studied using in vivo models and clinical trials to support its use as an antipyretic drug in Thai traditional medicine., (Copyright © 2020. Published by Elsevier B.V.)