1. Chemical composition and evaluation of the antinociceptive, antioxidant and antimicrobial effects of essential oil from Hymenaea cangaceira (Pinto, Mansano & Azevedo) native to Brazil: A natural medicine.
- Author
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Oliveira de Veras B, Melo de Oliveira MB, Granja da Silva Oliveira F, Queiroz Dos Santos Y, Saturnino de Oliveira JR, Lúcia de Menezes Lima V, Guedes da Silva Almeida JR, Maria do Amaral Ferraz Navarro D, Ribeiro de Oliveira Farias de Aguiar JC, Aguiar JDS, Gorlach-Lira K, Dias de Assis CR, Vanusa da Silva M, and Catarina de Souza Lopes A
- Subjects
- Acetic Acid toxicity, Analgesics chemistry, Analgesics isolation & purification, Animals, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents isolation & purification, Antioxidants chemistry, Antioxidants isolation & purification, Brazil, Disease Models, Animal, Drug Evaluation, Preclinical, Ethanol chemistry, Ethnopharmacology, Formaldehyde toxicity, Humans, Medicine, Traditional methods, Mice, Microbial Sensitivity Tests, Nociception drug effects, Oils, Volatile chemistry, Oils, Volatile therapeutic use, Pain chemically induced, Pain diagnosis, Pain drug therapy, Pain Measurement, Plant Oils chemistry, Plant Oils isolation & purification, Toxicity Tests, Acute, Analgesics pharmacology, Anti-Bacterial Agents pharmacology, Antioxidants pharmacology, Hymenaea chemistry, Oils, Volatile pharmacology, Plant Oils pharmacology
- Abstract
Ethnopharmacological Relevance: Hymenaea cangaceira Pinto, Mansano & Azevedo (Fabaceae) is a Brazilian medicinal plant widely known as "Jatobá". In folk medicine, it is used to treat infections, respiratory problems, rheumatism, antitumoral, inflammation and pain, however, no activity has been scientifically validated., Aim of the Study: This study investigated chemical composition of essential oil from Hymenaea cangaceira (EOHc), antimicrobial, antinociceptive and antioxidant activities besides protection against DNA damage and hemolysis., Material and Methods: The essential oil was obtained by hydrodistillation, and characterized by GC-MS and GC-FID. The evaluation of antimicrobial activity was performed by microdilution method. The evaluation of the antioxidant activity was performed using the radicals DPPH, ABTS, O
2 - and OH- , and the protection of DNA damage using plasmid pBR322. Different experimental models were used to evaluate the antinociceptive effect (acetic acid and formalin), and evaluate the mechanisms of action involved with pharmacological antagonists (naloxone, atropine and gibenclamide) in mice. The essential oil was evaluated for hemolysis on human erythrocytes., Results: The extraction of EOHc showed a yield of 0.18% on a dry basis, presenting high content of hydrocarbon sesquiterpenes (79.04%), high antioxidant activity and protect DNA from damage, besides presenting antifungal and antibacterial activity against Gram-positive and Gram-negative bacteria in vitro. It was found that the essential oil had no acute toxicity in mice up to 5000 mg/kg oral administration (o.a.), in addition to no hemolysis on human erythrocytes. The reduction of antinociceptive activity was 75%, with the opioid system as the mechanism of action., Conclusion: Our results validate the main activities by the traditional use attributed to H. cangaceira for antimicrobial and analgesic activity. In addition, the oil has a potent antioxidant activity, protecting the body against oxidative stress damage, adding new value to an endemic species not known to the industry., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2020
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