1. Role of the adjunctive antimicrobial photodynamic therapy to periodontal treatment at plasmatic oxidative stress and vascular behavior.
- Author
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Barin LM, Barcelos RCS, Vey LT, Pillusky FM, Palma VM, Kantorski KZ, Bürger ME, Maciel RM, and Danesi CC
- Subjects
- Animals, Anti-Infective Agents therapeutic use, Ascorbic Acid blood, Blood Vessels drug effects, Blood Vessels pathology, Blood Vessels radiation effects, Dental Scaling, Gingiva pathology, Gingiva radiation effects, Glutathione blood, Light, Male, Methylene Blue therapeutic use, Oxidative Stress radiation effects, Periodontitis drug therapy, Periodontitis radiotherapy, Photochemotherapy, Photosensitizing Agents therapeutic use, Rats, Rats, Wistar, Root Planing, Anti-Infective Agents pharmacology, Methylene Blue pharmacology, Oxidative Stress drug effects, Photosensitizing Agents pharmacology
- Abstract
Background: To evaluate for the first time in vivo the effects of methylene blue (MB) photosensitizer dissolved in ethanol in antimicrobial photodynamic therapy (aPDT) as adjuvant periodontal treatment, at plasmatic oxidative stress and vascular behavior in rat model., Methods: Wistar rats were divided into negative control (NC, no periodontitis) and positive control (PC, with periodontitis, without any treatment). The other groups had periodontitis and were treated with scaling and root planing (SRP); SRP+aPDT+MB dissolved in water (aPDT I); SRP+aPDT+MB dissolved in ethanol (aPDT II). The periodontitis was induced by ligature at the mandibular right first molar. At 7/15/30days, rats were euthanized, the plasma was used to determine oxidative stress parameters and gingival tissue for histomorphometric analysis., Results: PC showed higher thiobarbituric acid reactive substances levels in 7/15/30days. aPDT II was able to block the lipid peroxidation, especially between 15th and 30th days. Glutathione reduced levels were consumed in PC, aPDT I and II groups throughout the experiment. aPDT II increased the vitamin C levels which were restored in this group in the 30th day. aPDT II group showed the highest number of blood vessels., Conclusion: In summary, the aPDT with MB dissolved in ethanol provides better therapeutic responses in periodontitis treatment., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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