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Melatonin and metal resistant microbes can enhance plant defense responses against various abiotic stresses, but little is known about the combined effects of melatonin and chromium (Cr) resistant microbes on reducing Cr toxicity in wheat (Triticum aestivum L.). In current study, we examined the effects of combined application of melatonin (0, 1, 2 mM) and Bacillus subtilis (with and without inoculation) on wheat physio-biochemical responses and Cr uptake under different levels of Cr (0, 25, 50 and 100 mg Cr kg -1 DM soil). Chromium stress decreased the wheat growth, biomass, chlorophyll and relative water contents by causing oxidative damage in the form of overproduction of electrolyte leakage, hydrogen peroxide and malondialdehyde. However, foliar application of melatonin enhanced the plant growth, biomass and photosynthesis by alleviating the oxidative damage and Cr accumulation by plants. Melatonin significantly increased the enzymatic and non-enzymatic antioxidant activities as compared with respective control. Inoculation with microbes further enhanced the positive impacts of melatonin on wheat growth and reduced the Cr uptake by plants. Compared with non-inoculation and melatonin treatment, the inoculation with B. subtilis increased cholorophyll a by 27%, cholorophyll b by 49%, ascorbic acid in leaves by 50% and soluble proteins by 72% in wheat grwon with 50 mg Cr kg -1 DM soil. The application of B. subtilis reduced oxidative stress and Cr toxicity by transforming the Cr 6+ to Cr 3+ in shoots and roots of wheat. Furthermore, B. subtilis reduced the Cr 6+ uptake by wheat plants. The result of the present study revealed that the combined application of melatonin and B. subtilis might be a feasible approach aiming to reduce the Cr toxicity and its accumulation by wheat and probably in other plants., Competing Interests: Declaration of competing interest Authors declare no conflict of interest., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Here we compared the performance of four macrophytes namely Brachia mutica, Typha domingensis, Phragmites australis and Leptochala fusca, in bacterially assisted floating treatment wetlands (FTWs) for the clean-up of five trace metals (Fe, Mn, Ni, Pb, and Cr) from polluted river water. The river water was artificially spiked with reagent grade chemicals to increase the trace metal pollution. The macrophytes were planted in a polystyrene sheet to prepare FTWs, which were placed over the metal-contaminated river water. The consortium of five rhizospheric and endophytic bacterial strains, i.e., Aeromonas salmonicida, Pseudomonas indoloxydans, Bacillus cerus, Pseudomonas gessardii, and Rhodococcus sp., was inoculated support the natural remediation ability. We found a significant reduction in the metal content by all four macrophytes and the removal was significantly enhanced when bacterial inoculum was applied. The maximum removal was observed in FTWs planted with P. australis and inoculated with bacteria. In this treatment (T6) the Fe, Mn, Ni, Pb and Cr contents were reduced to 0.53, 0.20, 0.09, 1.04 and 0.07 mg L -1 after five weeks retention time. The bacterial inoculation sufficiently increased the plant biomass. All macrophytes depicted potential to uptake and translocate trace metals in the roots instead of shoots. The bacterial inoculation acclimatize the plants roots followed by shoots and enhanced the uptake of metals by macrophytes. This study emphasized the usefulness of macrophytes-bacteria mutualism in FTWs system for the remediation of trace metals. The similar systems may provide practical solutions for the remediation of trace metals of polluted river water., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Contamination of arid and semi-arid ecosystems by toxic heavy metals is a serious concern due to its impact on growth and productivity of crop and health risk through food chain. Therefore, the aims of this investigation were to study the impact of inorganic (i.e. nano hydroxyapatite, NHA and polyacrylamide, PAM) and organic (i.e. sugar beet factory lime, SBFL and biochar, BI) soil amendments on maize productivity grown in contaminated silty clay (i.e. fluvial, lacustrine) and sandy (i.e. marine) alkaline soils. In addition, the effect of those amendments on the content of heavy metals in plant organs and their fractions in alkaline soils at harvest as well as human health risk assessment were investigated. Application of amendments, particularly SBFL, followed by NHA and BI resulted in an improvement for maize growth and its productivity in comparison to PAM application and untreated soil. However, application of NHA significantly reduced the mobile fraction of Cd, Pb and Ni in soil and consequently in different maize organs, followed by application of SBFL and BI in comparison to untreated soil or soil treated with PAM. Additionally, transfer factors and health risk of metals were lesser when NHA and SBFL were applied into soil than those obtained from application of PAM. In conclusion, applications of NHA, SBFL and BI into contaminated alkaline soils with toxic heavy metals can be considered a vital option for ameliorating such soils from the view of environment and sustainable management in terms of heavy metals immobilization and reducing the metals content in plant organs., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Soil salinity and atmosphere temperature change have negative impacts on crop productivity and its quality and can pose a significant risk to soil properties in semi-arid regions. We conducted two field experiments in North (first zone) and South (second zone) of Egypt to investigate the effects of soil bagasse ash (10 ton ha -1 ), foliar thiourea (240 g ha -1 ) and their combination in comparison to the control treatment on saline soil properties and productivity and quality traits of wheat. All studied treatments were received the recommended rate of N, P and K fertilizations. Combination of soil bagasse ash and foliar thiourea application resulted in a significant improvement of most studied soil properties (i.e. EC, compaction, hydraulic conductivity, OM and available P, K, N contents) after harvest in comparison to other treatments in both of zones. Also, it enhanced growth and grain yield of wheat in terms of photosynthesis related attributes and yield components. Moreover, combination of soil bagasse ash and foliar thiourea application resulted in superior grain quality traits in terms of carbohydrate, fibre, protein and ash contents than separated application of soil bagasse ash, foliar thiourea or even control treatment. In conclusion, combination of soil bagasse ash and foliar thiourea application can be used as suitable option to enhance plant nutrition, wheat productivity and improve wheat grain quality and soil traits in saline soil as well as can alleviate heat stress., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Recycling nutrients saves energy and improves agricultural sustainability. Sewage sludge contains 2.6% P and 3.1% N, so the availability of these nutrients was investigated using four crops grown in either soil or sand. Further attention was paid to the role of mycorrhiza in improvement of nutrient availability. The content of heavy metals and metalloids in the feedstock was analyzed. Sewage sludge application resulted in greater biomass accumulation in ryegrass than comparable single applications of either synthetic fertilizer or digested sludge. Sewage sludge application resulted in more numerous mycorrhizal spores in soil and increased root colonization in comparison to synthetic fertilizer. All plants studied had mycorrhizal colonized roots, with the highest colonization rate in maize, followed by hemp. Sewage sludge application resulted in the highest P uptake in all soil-grown plants. In conclusion, sewage sludge application increased feedstock yield, provided beneficial use for organic wastes, and contributed to the sustainability of bioenergy feedstock production systems. It also improves the soil conditions and plant nutrition through colonization by mycorrhizal fungi as well as reducing leaching and need of synthetic fertilizers., (Copyright © 2013 Elsevier Ltd. All rights reserved.)

Sewage sludge is rich in essential plant nutrients, but its use is restricted for crop production due to the pollutants it contains, such as metalloids and heavy metals. Sludge is also very sticky and compact. Therefore, the objectives of this work were to evaluate (1) the impact of various amounts of sludge on bioenergy crop productivity and quality and (2) the use of peat as an adjuvant to reduce the stickiness, density and nutrient richness of the sludge. Three different applications of sludge were examined, high, low (50% of high) and low mixed with an equal volume of peat. The sludge-peat mix increased significantly leaf area and biomass accumulation of maize and hemp. High sludge and sludge-peat mix applications increased significantly the leaf area and biomass accumulation as well as the net photosynthesis of oilseed rape. High sludge application resulted in the highest heavy metal and metalloid accumulation in maize and hemp. Sludge-peat mix resulted in the highest heavy metal and metalloid accumulation in oilseed rape. However, the sludge-peat mix application provided the best feedstock quality in all three crops, since chloride, silicon and sulfur concentrations and ash content in plant material were the lowest of the three sludge treatments., (Copyright © 2012 Elsevier Ltd. All rights reserved.)