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Leptospirosis is a zoonotic disease caused by the bacteria of the genus Leptospira, which is responsible for substantial impacts on the economy, animal health, and public health. This disease has a global distribution and is particularly prevalent in Brazil. While leptospirosis can affect different animal species, including domestic ones such as dogs and cats, the role of dogs in its epidemiology is established and well-understood. Conversely, the role of domestic cats in the epidemiology of leptospirosis remains unclear, with a significant lack of studies elucidating the roles they play in the transmission of Leptospira spp. and the bacterial species they can host. Therefore, this study aimed to evaluate the presence of Leptospira spp. DNA in domestic cats and to assess the phylogenetic relationships of the identified microorganisms in the Central region of Rio Grande do Sul State (RS) in southern Brazil. The samples were evaluated for the presence of the gene lipL32 by polymerase chain reaction and sequencing of the amplified fragment, followed by phylogenetic analysis. DNA from Leptospira spp. was extracted from the kidney tissue of domestic cats. Pathogenic Leptospira spp. DNA was detected in 22.1 % (67/303) of the samples. Molecular analyses revealed the presence of L. borgpetersenii and L. interrogans in these animals. This study is the first to identify L. borgpetersenii and L. interrogans in domestic cats in RS, highlighting their potential role as bacterial reservoirs. Our findings provide valuable insights into the epidemiology of leptospirosis and can contribute to sanitary measures aimed at controlling and preventing the disease, ultimately protecting public health., Competing Interests: Declaration of Competing Interest None of the authors has any conflict of interest to declare., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Leptospirosis is a disease caused by Leptospira spp. responsible for considerable impacts on the public and animal health. In the past two decades, non-domesticated species of pets (unconventional pets) have become popular. However, the role of these unconventional pets on maintaining diseases still unclear. Therefore, the objective of this study was to survey the presence of Leptospira spp. DNA in unconventional pets. Samples of kidney tissues from 29 animals belonging to the Mammalia class (including Orders Carnivora, Lagomorpha and Rodentia) were analyzed for the presence of the gene lipL32. As a result, DNA of pathogenic Leptospira spp. from specie L. interrogans was detected in four (13,80%) of the analyzed samples: three from Oryctolagus cuniculus and one from Mesocricetus auratus. This study highlights the importance of epidemiological surveillance of leptospirosis, as it identified in species of unconventional pets, that may possibly act as reservoirs of Leptospira spp., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)