1. Soluble interleukin 6 (IL-6) receptor influences the expression of the protooncogene junB and the production of fibrinogen in the HepG2 human hepatoma cell line and primary rat hepatocytes.
- Author
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Igaz P, Tóth S, Rose-John S, Madurka I, Fejér G, Szalai C, and Falus A
- Subjects
- Animals, Carcinoma, Hepatocellular, Cells, Cultured, Humans, Liver cytology, Polymerase Chain Reaction methods, Proto-Oncogene Proteins c-jun genetics, RNA, Messenger, Rats, Solubility, Tumor Cells, Cultured, Fibrinogen biosynthesis, Gene Expression Regulation, Liver metabolism, Proto-Oncogene Proteins c-jun biosynthesis, Receptors, Interleukin-6 metabolism
- Abstract
Interleukin 6 (IL-6) belongs to a family of cytokines using receptors sharing a common signal-transducing chain, gp130 and containing a specific ligand-binding chain (IL-6R alpha). It was shown that both the membrane-bound and the soluble form (sIL-6R) of this ligand specific receptor chain occurs naturally. The soluble form of IL-6 receptor was found to be able to associate with the membrane-bound gp130 and to generate active IL-6 receptor complex capable of inducing signal transduction. This study on a human hepatoma cell line and primary rat hepatocytes examined how the effectiveness of IL-6 is modified by the presence of soluble IL-6 receptor and whether the sIL-6R in the absence of IL-6 acts on hepatocytes. The authors studied the gene expression of junB, a member of the Jun family of transcription factors, and the production of fibrinogen in response to IL-6 and sIL-6R. The data show that in hepatic cells, endogeneously expressing IL-6R, the IL-6 induced junB and fibrinogen expression is inhibited by the presence of sIL-6R. In addition we found that sIL-6R alone (in the absence of IL-6) induced junB mRNA expression, but had no effect on fibrinogen production.
- Published
- 1998
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