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The adsorption properties of 2,4-Dichlorophenoxyacetic acid (2,4-D) onto biochar, obtained through HCl-assisted hydrothermal carbonization process of Vateria indica fruits (VI-BC), were extensively studied using traditional and statistical physics approaches. The traditional adsorption investigations encompassed kinetics, equilibrium, and thermodynamics studies. Subsequently, the Hill statistical physics model was employed to interpret the mechanism. Also, artificial neural network (ANN) and adaptive neuro-fuzzy inference system (ANFIS) machine learning tools were successfully employed to model the adsorption data wherein both models had high prediction potential (R 2  > 0.99). The outcomes demonstrated that the produced VI-BC exhibited remarkable adsorptive traits, having a considerable specific surface area (111.54 m 2 /g), pore size (5.89 nm), a variety of functional groups, and appropriate attributes for efficiently adsorbing 2,4-D. For 10 mg/L 2,4-D, at pH 2.0 and with 0.3 g/L dose, an impressive 91.67% adsorption efficiency was achieved within a 120-min. Pseudo-second-order model aptly depicted the kinetic behavior of 2,4-D adsorption, while the Freundlich model provided a more accurate representation of the isotherms. 2,4-D maximum adsorption capacity stood at 131.39 mg/g at 303 K. The Hill statistical physics model elucidated that the adsorption primarily occurred via physisorption mechanisms, involving electrostatic attractions, π-π conjugation, and pore filling. This conclusion was further substantiated by post-adsorption characterization of the VI-BC. Thermodynamic analysis indicated that the interactions between VI-BC and 2,4-D were favorable, spontaneous, and exothermic. The calculated low energy of adsorption (1.255 kJ/mol) and ΔH° value (-20.49 kJ/mol) further supported physisorption as the dominant mechanism. In summary, this study underscores the significant potential of the newly developed biochar as a promising alternative material for efficiently removing the 2,4-D herbicide from polluted environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Benzo(a)pyrene, a five-ring polyaromatic hydrocarbon, originating from coal tar, crude oil, tobacco, grilled foods, car exhaust etc, is highly persistent in the environment. It has been classified as a Group I carcinogen, as on its ingestion in human body, diol epoxide metabolites are generated, which bind to DNA causing mutations and eventual cancer. Among various removal methods, bioremediation is most preferred as it is a sustainable approach resulting in complete mineralization of benzo(a)pyrene. Therefore, in this study, biodegradation of benzo(a)pyrene was performed by two strains of Pseudomonas, i. e WDE11 and WD23, isolated from refinery effluent. Maximum benzo(a)pyrene tolerance was 250 mg/L and 225 mg/L against Pseudomonas sp. WD23 and Pseudomonas sp. WDE11 correspondingly. Degradation rate constants varied between 0.0468 and 0.0513/day at 50 mg/L with half-life values between 13.5 and 14.3 days as per first order kinetics, while for 100 mg/L, the respective values varied between 0.006 and 0.007 L/mg. day and 15.28-16.67 days, as per second order kinetics. The maximum specific growth rate of strains WDE11 and WD23 was 0.3512/day and 0.38/day accordingly, while concentrations over 75 mg/L had an inhibitory effect on growth. Major degradation metabolites were identified as dihydroxy-pyrene, naphthalene-1,2-dicarboxylic acid, salicylic acid, and oxalic acid, indicating benzo(a)pyrene was degraded via pyrene intermediates by salicylate pathway through catechol meta-cleavage. The substantial activity of the catechol 2,3 dioxygenase enzyme was noted during the benzo(a)pyrene metabolism by both strains with minimal catechol 1,2 dioxygenase activity. This study demonstrates the exceptional potential of indigenous Pseudomonas strains in complete metabolism of benzo(a)pyrene., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

In the present study, ferric oxide nanoparticles impregnated with activated carbon from Ulva prolifera biomass (UPAC-Fe 2 O 3 ) were prepared and employed to remove 2,4-Dichlorophenoxyacetic acid (2,4-D) by adsorption. The UPAC-Fe 2 O 3 nanocomposite was characterized for its structural and functional properties by a variety of techniques. The nanocomposite had a jagged, irregular surface with pores due to uneven scattering of Fe 2 O 3 nanoparticles, whereas elemental analysis portrayed the incidence of carbon, oxygen, and iron. XRD analysis established the crystalline and amorphous planes corresponding to the iron oxide and carbon phase respectively. FT-IR analyzed the functional groups that confirmed the integration of Fe 2 O 3 nanoparticles onto nanocomposite surfaces. VSM and XPS studies uncovered the superparamagnetic nature and presence of carbon and Fe 2 O 3, respectively, in the UPAC-Fe 2 O 3 nanocomposite. While the surface area was 292.51 m 2 /g, the size and volume of the pores were at 2.61 nm and 0.1906 cm 3 /g, respectively, indicating the mesoporous nature and suitability of the nanocomposites that could be used as adsorbents. Adsorptive removal of 2,4-D by nanocomposite for variations in process parameters like pH, dosage, agitation speed, adsorption time, and 2,4-D concentration was studied. The adsorption of 2,4-D by UPAC-Fe 2 O 3 nanocomposite was monolayer chemisorption owing to Langmuir isotherm behavior along with a pseudo-second-order kinetic model. The maximum adsorption capacity and second order rate constant values were 60.61 mg/g and 0.0405 g/mg min respectively. Thermodynamic analysis revealed the spontaneous and feasible endothermic adsorption process. These findings confirm the suitability of the synthesized UPAC-Fe 2 O 3 nanocomposite to be used as an adsorbent for toxic herbicide waste streams., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Herein, the sustainable fabrication of magnetic iron oxide nanoadsorbent prepared with activated carbon of inedible Cynometra ramiflora fruit has been investigated. Activated carbon was obtained from phosphoric acid-treated C. ramiflora fruit, which was then utilized for the synthesis of magnetic nanocomposite (CRAC@Fe 2 O 3 ). The formed nanocomposite was a porous irregular dense matrix of amorphous evenly sized spherical nanoparticles, as visualized by FESEM, and also contained carbon, oxygen, iron, and phosphorous in its elemental composition. FT-IR spectrum depicted characteristic bands attributing to Fe-O, C-OH, C-N, CC, and -OH bonds. VSM and XRD results proved that CRAC@Fe 2 O 3 was superparamagnetic with a moderate degree of crystallinity and high saturation magnetization value (1.66 emu/g). Superior surface area, pore size, and pore volume of 766.75 m 2 /g, 2.11 nm, and 0.4050 cm 3 /g respectively were measured on BET analysis of CRAC@Fe 2 O 3 nanocomposite, indicating their suitability for use as an adsorbent. On application of this nanocomposite for adsorption of tetracycline, maximum removal of 95.78% of 50 ppm TC at pH 4, CRAC@Fe 2 O 3 0.4 g/L in 240 min. The adsorption of TC by CRAC@Fe 2 O 3 was confirmed as monolayer sorption by ionic interaction (R 2  = 0.9999) as it followed pseudo-second-order kinetics and Langmuir isotherm (R 2  = 0.9801). CRAC@Fe 2 O 3 showed a maximum adsorption capacity of 312.5 mg/g towards TC antibiotics indicating its potential for the treatment of antibiotic-contaminated samples. Since negative ΔG o and positive ΔH o and ΔS o values were obtained at all tested temperatures during the thermodynamic studies, the adsorption was confirmed to be endothermic, spontaneous, and feasible with an enhanced degree of randomness., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The final sinkers of polyaromatic hydrocarbons are water sources, where they undergo bioaccumulation and biomagnification, leading to adverse mutagenic, carcinogenic, and teratogenic effects on exposure in flora, fauna, and humans. Two indigenous strains, Pseudomonas sp. WDE11 and Pseudomonas sp. WD23, isolated from refinery effluent, degraded over 97.5% of benzo(a)fluorene (10 mg/L) in 7 days. On growth at concentration dependent amounts (50 mg/L and 100 mg/L), the degradation reduced to approximately 90% and 80% respectively in 56 days. Degradation kinetics was concentration dependent, as degradation followed first-order and second-order kinetics for 50 mg/L and 100 mg/L respectively. The half-life for degradation of benzo(a)fluorene ranged between 11.64 - 12.26 days and 13.11-14.5 days for strains WDE11 and WD23 respectively. The values of Andrew-Haldane kinetic parameters i.e. μ max , K s , and K i were 0.306 day -1 , 11.11 mg/L, and 120.41 mg/L for strain WDE11 respectively, while for strain WD23, the respective values were 0.312 day -1 , 9.97 mg/L, and 152 mg/L. Degradation metabolites were identified by their MS patterns as 3,4-dihydroxy fluorene, 2-(1-oxo-2,3-dihydro-1H-inden-2-yl) acetic acid, 3,4-dihydrocoumarin, salicylic acid, catechol, and oxalic acid. Metabolic pathway of degradation constructed, revealed that benzo(a)fluorene was metabolized via the formation of fluorene, further metabolized by salicylate pathway forming catechol. The catechol formed was degraded into simpler metabolites by meta-cleavage pathway, which was validated by catechol 2,3 dioxygenase enzyme activity., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Effect of oil spills on living forms demands for safe, ecofriendly and cost-effective methods to repair the damage. Pseudomonads have exceptional tolerance to xenobiotics and can grow at varied environmental conditions. This study aims at biosurfactant mediated degradation of petroleum crude oil by an indigenous Pseudomonas sp. WD23 in sea water. Pseudomonas sp. WD23 degraded 34% of petroleum crude oil (1.0% v/v) on supplementation of yeast extract (0.05 g/L) with glucose (1.0 g/L) in seawater. The strain produced a biosurfactant which was confirmed as a rhamnolipid (lipid: rhamnose 1:3.35) by FT-IR, LCMS and quantitative analysis. Produced rhamnolipid had low CMC (20.0 mg/L), emulsified petroleum oils (75-80%) and had high tolreance to varied conditions of pH, temperature and ionic strength. OFAT studies were performed to analyse the effect of petroleum crude oil, glucose, inoculum, yeast extract, pH, agitation speed and incubation time on degradation by Pseudomonas sp. WD23. Petroleum crude oil and glucose had significant effect on biodegradation, rhamnolipid production and growth, further optimized by central composite design. At optimum conditions of 3.414% v/v PCO and 6.53 g/L glucose, maximum degradation of 81.8 ± 0.67% was observed at pH 7.5, 100 RPM, 15.0% v/v inoculum in 28 days, with a 3-fold increase in biodegradation. GCMS analysis revealed degradation (86-100%) of all low and high molecular weight hydrocarbons present in petroleum crude oil. Hence, the strain Pseudomonas sp. WD23 can be effectively developed for management of oil spills in seas and oceans due to its excellent degradation abilities., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Heavy metals are dumped into the environment as a result of human activities, posing a serious threat to ecology and human health. Surface water, potable drinking water, potable groundwater, and majority of wastewater include their traces, due to which, their detection by nanoparticles has received a lot of attention in recent years. Although microorganisms are utilized for green nanoparticle production, plant materials have recently been explored because they are more environmentally friendly, easier to scale up, and require fewer specific growth conditions. The production and attributes of nanoparticles synthesized by plant mediation could be enhanced through design of experiments approach, extending their feasibility in the detection of heavy metals in polluted environmental samples. A concise review on green synthesis of silver and gold nanoparticles utilizing plant phytochemicals, its mechanism of synthesis along with significance of design of experiments for enhancement, and their use as heavy metal contamination detectors is presented in the current study., (Copyright © 2022 Elsevier Ltd. All rights reserved.)