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Neurotoxic insecticides are ubiquitous in aquatic ecosystems, frequently as part of complex mixtures. Freshwater gastropods are generally underrepresented in neurotoxicity evaluations and cumulative toxicity testing. This study investigates the behavioural and biochemical effects of acute exposures to the carbamate carbaryl, the organophosphate chlorpyrifos, and the neonicotinoid acetamiprid on the freshwater gastropod Chilina gibbosa. First, we evaluated behavioural neurotoxicity and cholinesterase (ChE), carboxylesterase (CE), and glutathione S-transferase (GST) activities in acute (48h) single-chemical exposures to increasing concentrations of carbaryl (0.5-500 μg L -1 ), chlorpyrifos (10-7500 μg L -1 ), and acetamiprid (1-10000 μg L -1 ). We then studied the effects of acute (48h) exposures to binary mixtures of carbaryl and chlorpyrifos equivalent to 0.5, 1, and 1.5 ChE 48h-IC 50 . None of the insecticides caused severe behavioural neurotoxicity, except for a significant lack of adherence by 5000 μg L -1 chlorpyrifos. Carbaryl caused concentration-dependent inhibition of ChEs (NOEC 5 μg L -1 ; 48h-IC 50 45 μg L -1 ) and CEs with p-nitrophenyl butyrate as substrate (NOEC 5 μg L -1 ; 48h-IC 50 37 μg L -1 ). Chlorpyrifos caused concentration-dependent inhibition of ChEs (NOEC 50 μg L -1 ; 48h-IC 50 946 μg L -1 ) but did not affect CEs (NOEC ≥7500 μg L -1 ). Carbaryl-chlorpyrifos mixtures inhibited ChEs additively, inhibited CEs with p-nitrophenyl butyrate, and did not affect behaviour. GST activity was not affected by single or mixture exposures. Acute exposure to acetamiprid did not affect any of the endpoints evaluated. This study provides new information on carbaryl, chlorpyrifos, and acetamiprid toxicity on C. gibbosa, relevant to improve gastropod representation in ecotoxicological risk assessment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)