1. Recent progress with FKBP-derived destabilizing domains.
- Author
-
Chu BW, Banaszynski LA, Chen LC, and Wandless TJ
- Subjects
- Amino Acid Motifs, Cell Cycle drug effects, Cyclin B metabolism, Cyclin B1, HeLa Cells, Humans, Structure-Activity Relationship, Tacrolimus Binding Proteins genetics, Tacrolimus Binding Proteins metabolism, Models, Molecular, Tacrolimus Binding Proteins chemistry
- Abstract
The FKBP-derived destabilizing domains are increasingly being used to confer small molecule-dependent stability to many different proteins. The L106P domain confers instability to yellow fluorescent protein when it is fused to the N-terminus, the C-terminus, or spliced into the middle of yellow fluorescent protein, however multiple copies of L106P do not confer greater instability. These engineered destabilizing domains are not dominant to endogenous degrons that regulate protein stability.
- Published
- 2008
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